Razvoj monoklonskih protiteles proti membranskim proteinom na modelu B-celičnega maturacijskega antigena : raziskovalni podatki testiranja s pretočno citometrijo, obravnavani v doktorskem delu

Kern, Petra (author), Kolenc, Ana (author), Kovač, Valerija (author), Čurin-Šerbec, Vladka (author)

B-celični maturacijski antigen (BCMA) je membranski protein, ki se specifično izraža na površini limfocitov B. BCMA je obetavna tarča za zdravljenje B-celičnih malignosti, zato smo želeli pripraviti protitelesa proti BCMA, ki bi se vezala na BCMA izražen na živih celicah. V ta namen smo s tehnologijo hibridomov pripravili širok nabor protiteles. Specifičnost in afiniteto protiteles smo testirali s pretočno citometrijo. Za testiranje smo uporabljali pretočni citometer MACS Quant in slikovni pretočni citometer ImageStream. S pridobljenimi protitelesi smo barvali različne komercialno dostopne celične linije, ki se razlikujejo v intenziteti izražanja BCMA.

• Language: Slovenian
• Keywords: B-celični maturacijski antigen, tehnologija hibridomov, monoklonska protitelesa, membranski proteini, pretočna citometrija
• Work type: Research data
• Typology: 2.20 - Research data
• Geographic coverage: Ljubljana, Slovenija
• Temporal coverage: Od/From 2024-03-26 do/to 2026-02-18
• Organization: MF - Faculty of Medicine
• Year: 2026
• PID: 20.500.12556/RUL-190015
• Data col. methods: Experiment: Laboratory
• Publication date in RUL: 22.10.2026

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: English
• Title: Development of monoclonal antibodies against B-cell maturation antigen as a model for production of antibodies against membrane proteins : research data from flow cytometry experiments, underlying the doctoral dissertation

Abstract

B-cell maturation antigen (BCMA) is a membrane protein specifically expressed on the surface of B lymphocytes. BCMA is a promising target for the treatment of B-cell malignancies; therefore, we aimed to develop antibodies against BCMA that would bind to BCMA expressed on live cells. To this end, we generated a broad panel of antibodies using hybridoma technology. The specificity and affinity of the antibodies were assessed by flow cytometry. A MACS Quant flow cytometer and an ImageStream imaging flow cytometer were used for testing. The antibodies obtained were used to stain various commercially available cell lines that differ in the level of BCMA expression.

• Keywords: B-cell maturation antigen, hybridoma technology, monoclonal antibodies, membrane proteins, flow cytometry

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P4-0176
• Name: Sintezna biologija in imunologija

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: J7-4640
• Name: Inovativna imunoterapija raka preko CAR T celic (CARRS)