20.500.12556/RUL-190015 Razvoj monoklonskih protiteles proti membranskim proteinom na modelu B-celičnega maturacijskega antigena raziskovalni podatki testiranja s pretočno citometrijo, obravnavani v doktorskem delu Development of monoclonal antibodies against B-cell maturation antigen as a model for production of antibodies against membrane proteins research data from flow cytometry experiments, underlying the doctoral dissertation B-celični maturacijski antigen (BCMA) je membranski protein, ki se specifično izraža na površini limfocitov B. BCMA je obetavna tarča za zdravljenje B-celičnih malignosti, zato smo želeli pripraviti protitelesa proti BCMA, ki bi se vezala na BCMA izražen na živih celicah. V ta namen smo s tehnologijo hibridomov pripravili širok nabor protiteles. Specifičnost in afiniteto protiteles smo testirali s pretočno citometrijo. Za testiranje smo uporabljali pretočni citometer MACS Quant in slikovni pretočni citometer ImageStream. S pridobljenimi protitelesi smo barvali različne komercialno dostopne celične linije, ki se razlikujejo v intenziteti izražanja BCMA. B-cell maturation antigen (BCMA) is a membrane protein specifically expressed on the surface of B lymphocytes. BCMA is a promising target for the treatment of B-cell malignancies; therefore, we aimed to develop antibodies against BCMA that would bind to BCMA expressed on live cells. To this end, we generated a broad panel of antibodies using hybridoma technology. The specificity and affinity of the antibodies were assessed by flow cytometry. A MACS Quant flow cytometer and an ImageStream imaging flow cytometer were used for testing. The antibodies obtained were used to stain various commercially available cell lines that differ in the level of BCMA expression. B-celični maturacijski antigen tehnologija hibridomov monoklonska protitelesa membranski proteini pretočna citometrija B-cell maturation antigen hybridoma technology monoclonal antibodies membrane proteins flow cytometry true false false Slovenski jezik Angleški jezik Ljubljana, Slovenija Od/From 2024-03-26 do/to 2026-02-18 Raziskovalni podatki 2026-10-20 13:07:32 2026-10-22 22:09:01 2026-10-23 08:21:41 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 README.txt README.txt README 1 51828A8E2C46EFBE76E8F8C46C90F705 fa49c8b3444c3f57634f995c4ff9c3318727e66ea034ef88790d0cbf228a5f63 9b2a3610-cd2b-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251049 PREBERI_ME.txt PREBERI_ME.txt PREBERI ME 1 8EB2D0F13E9445907111B81A3ACE255C 83d7bf2fc7e3f4c1bf43363e61b56be46bae81728de40aa8e28dea6832033831 9bf98702-cd2b-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251050 Pretocna_citometrija.zip Pretocna_citometrija.zip Pretočna citometrija 1 4967D94B7FE736B78597BBC7E692ECFF 4d72f6ce0e898de9c50ea186a013015241be28bbd24663eb56a33edee79607b7 9c7d2834-cd2b-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=251051 Medicinska fakulteta Poskus: Laboratorij 0 0 0