In this thesis, we investigated the ability of the fungi Hypoxylon fragiforme, Pleurotus ostreatus and Gloeophyllum trabeum to remove paracetamol (PCT) from a liquid PDB medium. The aim of the study was to compare the efficacy of two white rot fungi and one brown rot fungus, and to assess the effect of the timing of paracetamol addition. We used the commercial preparation LEKADOL 500 mg at an initial concentration of 1000 mg/L. The experiment was carried out in three biological replicates using two approaches: paracetamol was added to seven-day-old cultures or simultaneously with inoculation. Changes in paracetamol concentration in the liquid phase were monitored using HPLC, whilst culture growth was assessed qualitatively by photography. In pre-cultured P. ostreatus cultures, a paracetamol peak was not detected in any of the samples, whereas in H. fragiforme it was detected from the fifth day onwards. In G. trabeum, the concentration decreased from 555.30 mg/L on the third day to 242.94 mg/L on the fifth day, whilst on the tenth day a peak was detected in only one replicate. With simultaneous addition, no peak was detected in any of the samples for H. fragiforme; the concentration in P. ostreatus was 27.52 mg/L after 14 days, and 711.81 mg/L for G. trabeum. The results support the greater efficacy of white rot fungi; however, the reduction in concentration in the liquid phase does not prove complete biodegradation, as biosorption, paracetamol in the biomass and transformation products were not determined.
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