20.500.12556/RUL-186881 Biološka razgradnja paracetamola s pomočjo različnih vrst gliv bele in rjave trohnobe Biological Degradation of Paracetamol Using Different White-Rot and Brown-Rot Fungal Species V diplomskem delu smo proučevali sposobnost gliv Hypoxylon fragiforme, Pleurotus ostreatus in Gloeophyllum trabeum za odstranjevanje paracetamola (PCT) iz tekočega PDB-gojišča. Namen raziskave je bil primerjati učinkovitost dveh gliv bele trohnobe in ene glive rjave trohnobe ter oceniti vpliv časa dodatka paracetamola. Uporabili smo komercialni pripravek LEKADOL 500 mg v začetni koncentraciji 1000 mg/L. Poskus smo izvedli v treh bioloških ponovitvah po dveh pristopih: paracetamol smo dodali sedem dni starim kulturam ali sočasno z inokulacijo. Spremembo koncentracije paracetamola v tekoči fazi smo spremljali z metodo HPLC, rast kultur pa kvalitativno s fotografiranjem. Pri predhodno vzgojenih kulturah P. ostreatus vrh paracetamola ni bil zaznan pri nobenem vzorčenju, pri H. fragiforme od petega dne dalje. Pri G. trabeum se je koncentracija zmanjšala s 555,30 mg/L tretji dan na 242,94 mg/L peti dan, deseti dan je bil vrh zaznan le v eni ponovitvi. Pri sočasnem dodatku pri H. fragiforme vrh ni bil zaznan pri nobenem vzorčenju, koncentracija pri P. ostreatus je po 14 dneh znašala 27,52 mg/L, pri G. trabeum pa 711,81 mg/L. Rezultati podpirajo večjo učinkovitost gliv bele trohnobe, vendar zmanjšanje koncentracije v tekoči fazi ne dokazuje popolne biodegradacije, saj biosorpcija, paracetamol v biomasi in transformacijski produkti niso bili določeni. In this thesis, we investigated the ability of the fungi Hypoxylon fragiforme, Pleurotus ostreatus and Gloeophyllum trabeum to remove paracetamol (PCT) from a liquid PDB medium. The aim of the study was to compare the efficacy of two white rot fungi and one brown rot fungus, and to assess the effect of the timing of paracetamol addition. We used the commercial preparation LEKADOL 500 mg at an initial concentration of 1000 mg/L. The experiment was carried out in three biological replicates using two approaches: paracetamol was added to seven-day-old cultures or simultaneously with inoculation. Changes in paracetamol concentration in the liquid phase were monitored using HPLC, whilst culture growth was assessed qualitatively by photography. In pre-cultured P. ostreatus cultures, a paracetamol peak was not detected in any of the samples, whereas in H. fragiforme it was detected from the fifth day onwards. In G. trabeum, the concentration decreased from 555.30 mg/L on the third day to 242.94 mg/L on the fifth day, whilst on the tenth day a peak was detected in only one replicate. With simultaneous addition, no peak was detected in any of the samples for H. fragiforme; the concentration in P. ostreatus was 27.52 mg/L after 14 days, and 711.81 mg/L for G. trabeum. The results support the greater efficacy of white rot fungi; however, the reduction in concentration in the liquid phase does not prove complete biodegradation, as biosorption, paracetamol in the biomass and transformation products were not determined. paracetamol biološka razgradnja mikoremediacija glive bele trohnobe glive rjave trohnobe farmacevtska onesnaževala tekoče glivne kulture HPLC paracetamol biodegradation mycoremediation white-rot fungi brown-rot fungi pharmaceutical pollutants liquid fungal cultures HPLC true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2026-09-06 08:15:37 2026-09-06 08:15:41 2026-09-09 15:27:36 0000-00-00 00:00:00 2026 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 287176 290556163 61119.pdf 61119.pdf 1 B8FBCB4170F874603A3DD62B6F9E9721 43df85f1735638d1f01569ccbdfcea9b35494352bee6d1805657d0afdc0e620f 62039c9f-a9ba-11f1-8bc5-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=246263 Biotehniška fakulteta 0 0 0