This thesis presents the importance of producing virus-free planting material of cultivated strawberry (Fragaria × ananassa Dutch.) for modern, high-quality strawberry production. Due to vegetative propagation, plants are highly susceptible to virus accumulation, which can significantly reduce yield and fruit quality, even when infections remain asymptomatic. The thesis describes key viruses affecting this species, including SMYEV, SCV, SMoV, and SVBV, as well as their modes of transmission and economic impact. Particular attention is given to methods of virus detection, especially serological (ELISA) and molecular techniques (PCR, qPCR, RT-PCR, LAMP, RPA), with emphasis on their sensitivity, specificity, and practical use. The thesis also explores advanced approaches such as next-generation sequencing (NGS) and CRISPR-based diagnostics. In the context of virus elimination, techniques such as meristem culture, thermotherapy, and cryotherapy are evaluated, with studies confirming that the combination of thermotherapy and meristem culture is among the most effective strategies for obtaining virus-free plants. Such biotechnological approaches are essential for ensuring healthy propagation material and mitigating the negative impact of viral infections in commercial strawberry production.
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