20.500.12556/RUL-172925 Pridobivanje brezvirusnega sadilnega materiala žlahtnega jagodnjaka (Fragaria x ananassa Dutch.) in vitro Production of virus free planting material of strawberry (Fragaria x ananassa Dutch.) in vitro V diplomski nalogi je predstavljen pomen pridobivanja brezvirusnega sadilnega materiala žlahtnega jagodnjaka (Fragaria × ananassa Dutch.) za sodobno in kakovostno pridelavo jagod. Zaradi vegetativnega razmnoževanja so rastline zelo občutljive za kopičenje virusnih okužb, ki lahko bistveno zmanjšajo pridelek in kakovost plodov, tudi če okužbe ne povzročajo vidnih simptomov. Opisani so glavni virusi, ki okužujejo to vrsto, med njimi SMYEV, SCV, SMoV in SVBV, njihove poti prenosa ter gospodarski vpliv. Poseben poudarek je namenjen metodam za zaznavanje virusov, zlasti serološkim (ELISA) in molekularnim tehnikam (PCR, qPCR, RT-PCR, LAMP, RPA), ki so izpostavljene kot občutljive, specifične in primerne za rutinsko uporabo. Predstavljene so tudi napredne diagnostične metode, kot sta sekvenciranje naslednje generacije (NGS) in tehnologije CRISPR. Na področju odstranjevanja virusov so obravnavane metode, kot so kultura meristemov, termoterapija in krioterapija, pri čemer se kot najučinkovitejša strategija izkaže kombinacija termoterapije in kulture meristemov. Takšni biotehnološki pristopi so ključni za zagotavljanje zdravega sadilnega materiala in dolgoročno zmanjšanje negativnih učinkov virusnih okužb v komercialni pridelavi jagod. This thesis presents the importance of producing virus-free planting material of cultivated strawberry (Fragaria × ananassa Dutch.) for modern, high-quality strawberry production. Due to vegetative propagation, plants are highly susceptible to virus accumulation, which can significantly reduce yield and fruit quality, even when infections remain asymptomatic. The thesis describes key viruses affecting this species, including SMYEV, SCV, SMoV, and SVBV, as well as their modes of transmission and economic impact. Particular attention is given to methods of virus detection, especially serological (ELISA) and molecular techniques (PCR, qPCR, RT-PCR, LAMP, RPA), with emphasis on their sensitivity, specificity, and practical use. The thesis also explores advanced approaches such as next-generation sequencing (NGS) and CRISPR-based diagnostics. In the context of virus elimination, techniques such as meristem culture, thermotherapy, and cryotherapy are evaluated, with studies confirming that the combination of thermotherapy and meristem culture is among the most effective strategies for obtaining virus-free plants. Such biotechnological approaches are essential for ensuring healthy propagation material and mitigating the negative impact of viral infections in commercial strawberry production. Fragaria x ananassa detekcija virusov odstranjevanje virusov kultura meristemov termoterapija kemoterapija krioterapija Fragaria x ananassa virus detection virus elimination meristem culture thermotherapy chemotherapy cryotherapy true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2025-09-12 07:19:03 2025-09-12 07:19:05 2025-10-28 03:47:26 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 263664 248996867 51058.pdf 51058.pdf 1 E12B8C6652FAAEB353F5E06D2FFC3FBA b751788d8aff225c138fa245df7144a11e2e531d8eb58ec5b19483be6cb64034 d6183e5e-8f97-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=217059 Biotehniška fakulteta 0 0 0