Biopharmaceuticals play a crucial role in modern medicine, as they offer innovative therapeutic options for treating various diseases. Their use is expanding into different medical disciplines, highlighting their sustainability and significance in clinical practice. However, the production of biopharmaceutical products is a complex and demanding process. For example, chromatographic methods are almost indispensable in the downstream processes for isolation of monoclonal antibodies. The high cost of chromatographic carriers often causes very high economic burden. In the scope of this project, we investigated whether hybrid depth filters are a suitable alternative to traditional chromatographic carriers. Based on the comparison of both approaches, and the results of our experiments, by measuring target protein concentration, host cell proteins (HCP), protein aggregates, and DNA, we concluded that the performance of hybrid depth filters is comparable to some steps of chromatographic separation. Hybrid depth filters have shown great potential as a standalone step or as a complementary step to classical chromatographic separation.
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