20.500.12556/RUL-171966 Ovrednotenje učinkovitosti hibridnih globinskih filtrov v procesu čiščenja monoklonskih protiteles. Evaluation of the performance of hybrid depth filters in the purification of monoclonal antibodies Biofarmacevtske učinkovine igrajo ključno vlogo v sodobni medicini, saj predstavljajo inovativne terapevtske možnosti za zdravljenje različnih bolezni. Njihova uporaba se širi v različne medicinske discipline, kar kaže na njihovo trajnost in pomembnost v klinični praksi. Vendar pa je proizvodnja biofarmacevtskih izdelkov kompleksen in zahteven proces. Pri izolaciji monoklonskih protiteles si največkrat pomagamo s kromatografskimi metodami. Zaradi visokih stroškov kromatografskih stacionarnih faz so procesi čiščenja in posledično končna cena produkta ekonomsko neugodni. V obsegu naše raziskave smo preverjali, ali so lahko hibridni globinski filtri primerna alternativa tradicionalnim kromatografskim stopnjam čiščenja. Na podlagi primerjave obeh tehnik in analitičnih rezultatov merjenja koncentracije ciljnih proteinskih produktov, beljakovin gostiteljskih celic (HCP), beljakovinskih agregatov in DNK smo ugotovili, da dajejo metode hibridnih globinskih filtrov primerljive rezultate v primerjavi z nekaterimi običajnimi kromatografskimi metodami. Rezultati naših raziskav kažejo, da imajo hibridni globinski filtri velik potencial kot samostojen korak ali kot dopolnilni korak h klasični kromatografski separaciji. Biopharmaceuticals play a crucial role in modern medicine, as they offer innovative therapeutic options for treating various diseases. Their use is expanding into different medical disciplines, highlighting their sustainability and significance in clinical practice. However, the production of biopharmaceutical products is a complex and demanding process. For example, chromatographic methods are almost indispensable in the downstream processes for isolation of monoclonal antibodies. The high cost of chromatographic carriers often causes very high economic burden. In the scope of this project, we investigated whether hybrid depth filters are a suitable alternative to traditional chromatographic carriers. Based on the comparison of both approaches, and the results of our experiments, by measuring target protein concentration, host cell proteins (HCP), protein aggregates, and DNA, we concluded that the performance of hybrid depth filters is comparable to some steps of chromatographic separation. Hybrid depth filters have shown great potential as a standalone step or as a complementary step to classical chromatographic separation. Hibridni globinski filtri kromatografija monoklonsko protitelo zaključni procesi 3M™ Polisher ST filter Hybrid depth filters chromatography monoclonal antibody downstream process 3M™ Polisher ST filter true false false Slovenski jezik Angleški jezik Magistrsko delo/naloga 2025-09-05 07:15:13 2025-09-05 07:15:35 2025-09-28 04:03:27 0000-00-00 00:00:00 2025 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 262252 247828483 50965.pdf 50965.pdf 1 AB2434490DC3F345C348071FBFE8FA31 dcd90e3af5b54b26bf7217e5756927a34550da875e0a0812f825d8e0febbc8b8 3d9be08f-8a17-11f0-9328-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=215679 Biotehniška fakulteta 0 0 0