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Design, synthesis and evaluation of a novel fluorescent probe for competitive fluorescence polarization assay to screen galectin-8 inhibitors
ID Purić, Edvin (Author), ID Marinovich, Marina (Author), ID Kojek, Zala (Author), ID Kahl-Knutson, Barbro (Author), ID Leffler, Hakon (Author), ID Nilsson, Ulf (Author), ID Anderluh, Marko (Author), ID Mravljak, Janez (Author)

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Abstract
In the present work, we describe the design, synthesis and evaluation of a galectin-8-binding fluorescent probe designed for a competitive fluorescence polarization (FP) assay for screening new galectin-8N inhibitors. The probe was characterized for its photophysical properties and its binding affinity for galectin-8N was determined by using FP. We evaluated the probe in a competitive FP assay with three known galectin-8N inhibitors and demonstrated its suitability for high-throughput screening.

Language:English
Keywords:fluorescence polarisation assay, carbohydrates, lectins, galectin-8
Work type:Article
Typology:1.03 - Other scientific articles
Organization:FFA - Faculty of Pharmacy
Publication version:Version of Record
Year:2025
Number of pages:Str. 273-282
Numbering:Vol. 75, iss. 2
PID:20.500.12556/RUL-169974 This link opens in a new window
UDC:615.4:54
ISSN on article:1846-9558
DOI:10.2478/acph-2025-0010 This link opens in a new window
COBISS.SI-ID:233259779 This link opens in a new window
Publication date in RUL:30.06.2025
Views:297
Downloads:63
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Record is a part of a journal

Title:Acta pharmaceutica
Shortened title:Acta pharm.
Publisher:Croatian Pharmaceutical Society
ISSN:1846-9558
COBISS.SI-ID:3817585 This link opens in a new window

Licences

License:CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
Link:http://creativecommons.org/licenses/by-nc-nd/4.0/
Description:The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

Language:Slovenian
Keywords:fluorescenčni polarizacijski test, ogljikovi hidrati, lektini, galektin-8, farmacevtska kemija

Projects

Funder:EC - European Commission
Project number:765581
Name:Multidisciplinary European Joint Doctorate in the Design and Development of Glyco Drugs
Acronym:PhD4GlycoDrug
Funder:Other - Other funder or multiple funders
Project number:2020-03317
Name:The Swedish Research Council
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:P1-0208
Name:Farmacevtska kemija: načrtovanje, sinteza in vrednotenje učinkovin
Funder:ARIS - Slovenian Research and Innovation Agency
Project number:J1-50026
Name:Vloga novega sekvenčnega motiva bogatega z alanini pri kondenzaciji RNA-vezavnih proteinov
Funder:Other - Other funder or multiple funders
Project number:CA18103
Name:Innovation with Glycans: new frontiers from synthesis to new biological targets (INNOGLY)
Funder:Other - Other funder or multiple funders
Project number:CA18132
Name:Functional Glyconanomaterials for the Development of Diagnostics and Targeted Therapeutic Probes (GLYCONanoPROBES)
Funder:Other - Other funder or multiple funders
Funding programme:Ministrstvo za izobraževanje, znanost in šport
Project number:MR51882
Name:Ministry of Education, Science, and Sport (MIZŠ) and the European Regional Development Fund OP20.05187 RI-SI-EATRIS

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