20.500.12556/RUL-169974 Design, synthesis and evaluation of a novel fluorescent probe for competitive fluorescence polarization assay to screen galectin-8 inhibitors In the present work, we describe the design, synthesis and evaluation of a galectin-8-binding fluorescent probe designed for a competitive fluorescence polarization (FP) assay for screening new galectin-8N inhibitors. The probe was characterized for its photophysical properties and its binding affinity for galectin-8N was determined by using FP. We evaluated the probe in a competitive FP assay with three known galectin-8N inhibitors and demonstrated its suitability for high-throughput screening. fluorescence polarisation assay carbohydrates lectins galectin-8 fluorescenčni polarizacijski test ogljikovi hidrati lektini galektin-8 farmacevtska kemija true false true Angleški jezik Slovenski jezik Članek v reviji 2025-06-30 09:32:06 2025-06-30 09:32:08 2025-07-01 03:55:47 0000-00-00 00:00:00 2025 0 0 Str. 273-282 iss. 2 Vol. 75 2025 0000-00-00 Zaloznikova NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 615.4:54 1846-9558 10.2478/acph-2025-0010 233259779 27325.pdf 27325.pdf 1 CA37944453C1D998A822176B1B03382B df2e083b0059f5b15ca3b3254e9fd43878715cc5cacba02d5c37fb50828b75e2 e7d90867-5582-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211856 https://acta.pharmaceutica.farmaceut.org/wp-content/uploads/2025/06/27325.pdf 1 7170147f-5582-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211855 Fakulteta za farmacijo 0 0 0