Phenolics and cyanogenic glycosides in the ethanolic extract of black cherry Prunus serotina Ehrh. fruits

Brozdowski, Jakub (author), Veberič, Robert (author), Mikulič Petkovšek, Maja (author)

Black cherry is an invasive alien plant species that has spread throughout Europe. Various meth− ods are used to control its population including the removal of Prunus serotina biomass from forests. Black cherry trees bear fruit at a remarkably young age, usually between 3 and 5 years old, and produce an abundance of small black fruits that resemble cherries. Harvesting these fruits can help reduce the growth of the population. Harvested fruits can also be processed to extract valuable secondary plant substances, especially phenolics. This study compares the success of one and two week ethanolic extraction methods of black cherry fruit from two growing locations. To determine the content of bioactive compounds in fruit, the biomass fruit was extracted in ethanol solution. Following extraction, the content of bioactive compounds was determined using high−performance liquid chromatography (HPLC) equipped with two detectors. A mass spectrometry detector (MS$^n$) was used for qualitative analysis, while a diode array detector (DAD) was employed for quantitative determination. In total between 8614 and 10780 µg/ml of phenolics were extracted. The results show that the content of some groups of phenolics was influenced by time, namely flavonols and anthocyanins, while the content of flavanones and derivatives of hydroxycinnamic acid was influenced by the location of the harvest. When the fruits were soaked in alcohol for two weeks, the content of cyanogenic glycosides was not influ− enced by time or location. Cyanogenic glycosides content after two weeks of soaking in alcohol ranged from 31.6 to 32.9 µg/ml.

• Language: English
• Keywords: bioactive compounds, phenolics, cyanogenic glycosides, extraction, invasive plant species, invasive plants, forests, Black cherry, Prunus serotine, Europe
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: str. 169-178
• Numbering: Vol. 169, iss. 3
• PID: 20.500.12556/RUL-169916
• UDC: 543.2:582.639.41
• ISSN on article: 0039-7660
• DOI: 10.26202/sylwan.2024067
• COBISS.SI-ID: 239206915
• Publication date in RUL: 16.06.2025

Record is a part of a journal

• Title: Sylwan
• Publisher: Polskie Naukowe Towarzystwo Leśne
• ISSN: 0039-7660
• COBISS.SI-ID: 13507355

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Title: Zawartość związków fenolowych oraz glikozydów cyjanogennych w etanolowym ekstrakcie z owoców czeremchy amerykańskiej Prunus serotina Ehrh.
• Keywords: bioaktivne spojine, fenoli, cianogeni glikozidi, ekstrakcija, invazivne rastlinske vrste, invazivne rastline, pozna čremsa, Prunus serotina, Evropa

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P4-0013
• Name: Hortikultura