20.500.12556/RUL-169916 Phenolics and cyanogenic glycosides in the ethanolic extract of black cherry Prunus serotina Ehrh. fruits Zawartość związków fenolowych oraz glikozydów cyjanogennych w etanolowym ekstrakcie z owoców czeremchy amerykańskiej Prunus serotina Ehrh. Black cherry is an invasive alien plant species that has spread throughout Europe. Various meth− ods are used to control its population including the removal of Prunus serotina biomass from forests. Black cherry trees bear fruit at a remarkably young age, usually between 3 and 5 years old, and produce an abundance of small black fruits that resemble cherries. Harvesting these fruits can help reduce the growth of the population. Harvested fruits can also be processed to extract valuable secondary plant substances, especially phenolics. This study compares the success of one and two week ethanolic extraction methods of black cherry fruit from two growing locations. To determine the content of bioactive compounds in fruit, the biomass fruit was extracted in ethanol solution. Following extraction, the content of bioactive compounds was determined using high−performance liquid chromatography (HPLC) equipped with two detectors. A mass spectrometry detector (MS$^n$) was used for qualitative analysis, while a diode array detector (DAD) was employed for quantitative determination. In total between 8614 and 10780 µg/ml of phenolics were extracted. The results show that the content of some groups of phenolics was influenced by time, namely flavonols and anthocyanins, while the content of flavanones and derivatives of hydroxycinnamic acid was influenced by the location of the harvest. When the fruits were soaked in alcohol for two weeks, the content of cyanogenic glycosides was not influ− enced by time or location. Cyanogenic glycosides content after two weeks of soaking in alcohol ranged from 31.6 to 32.9 µg/ml. bioactive compounds phenolics cyanogenic glycosides extraction invasive plant species invasive plants forests Black cherry Prunus serotine Europe bioaktivne spojine fenoli cianogeni glikozidi ekstrakcija invazivne rastlinske vrste invazivne rastline pozna čremsa Prunus serotina Evropa true false true Angleški jezik Slovenski jezik Članek v reviji 2025-06-16 11:26:56 2025-06-16 11:27:25 2025-06-17 04:24:58 0000-00-00 00:00:00 2025 0 0 str. 169-178 iss. 3 Vol. 169 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 543.2:582.639.41 0039-7660 10.26202/sylwan.2024067 239206915 2025_03_169.pdf 2025_03_169.pdf 1 76E1808639B4B28373F679DB1B357D09 3e9209df9a348c9ec9a2de9bdb23ede13a7016962b9f87e750748534b80471f8 c44c432e-4a92-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211692 https://sylwan-journal.pl/apex/f?p=sylwan:10:::NO::P10_NAZWA_PLIKU,P10_ARTYKUL,P10_ZESZYT_NEW:30858908047808042/2025_03_169au.pdf,2024067,2025_3 1 9513e2f1-4a92-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=211691 Biotehniška fakulteta 0 0 0