Evaluation of the effects of estrogens on endometrial cancer cells of different grades

Pavlič, Renata (author), Pušić Novak, Maja (author), Lanišnik-Rižner, Tea (author)

Endometrial cancer (EC) is the most common gynecological malignancy in the Western world. The molecular basis and effects of various agents are frequently studied in model EC cell lines, but the most commonly used cell lines Ishikawa, HEC-1-A, RL95-2 and KLE have not been thoroughly and systematically investigated. We characterized EC cell lines of different grades by reassessing the expression of estrogen receptors ERα, ERβ, and GPER by qPCR and Western blot and investigated the effects of estrogens, estrone-sulfate, estrone and estradiol on their proliferation, migration, and clonogenicity. Estradiol promoted the proliferation of grade 1 Ishikawa EC cells and grade 2 RL95-2 cells. Estrone and estrone sulfate also stimulated the proliferation of Ishikawa, showed a tendency to increase the proliferation of HEC-1-A and RL95-2 cells, but decreased the proliferation of KLE. Estrogens had no effect on the migration and clonogenicity of these four EC cell lines, however, there was a trend toward a smaller colony area for cells incubated with higher estrogen concentrations. We have previously shown that in EC estradiol forms from inactive estrone sulfate via the sulfatase pathway. This study showed that estrogens significantly promote the proliferation of grade 1 Ishikawa EC cells, and grade 2 RL95-2 and decrease the proliferation of grade 3 KLE cells. These differences in proliferation were associated with ERα positivity of Ishikawa cells and GPER expression in other cells.

• Language: English
• Keywords: clonogenicity, endometrial cancer, estradiol, estrone, estrone-sulfate, migration, proliferation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: 13 str.
• Numbering: Vol. 251, art. 106762
• PID: 20.500.12556/RUL-168993
• UDC: 61:577.2
• ISSN on article: 1879-1220
• DOI: 10.1016/j.jsbmb.2025.106762
• COBISS.SI-ID: 234835459
• Publication date in RUL: 06.05.2025

Record is a part of a journal

• Title: The journal of steroid biochemistry and molecular biology
• Shortened title: J. steroid biochem. mol. biol.
• Publisher: Elsevier
• ISSN: 1879-1220
• COBISS.SI-ID: 57401603

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: klonogenost, rak endometrija, estradiol, estron, estron-sulfat, migracije, širjenje