20.500.12556/RUL-168993 Evaluation of the effects of estrogens on endometrial cancer cells of different grades Endometrial cancer (EC) is the most common gynecological malignancy in the Western world. The molecular basis and effects of various agents are frequently studied in model EC cell lines, but the most commonly used cell lines Ishikawa, HEC-1-A, RL95-2 and KLE have not been thoroughly and systematically investigated. We characterized EC cell lines of different grades by reassessing the expression of estrogen receptors ERα, ERβ, and GPER by qPCR and Western blot and investigated the effects of estrogens, estrone-sulfate, estrone and estradiol on their proliferation, migration, and clonogenicity. Estradiol promoted the proliferation of grade 1 Ishikawa EC cells and grade 2 RL95-2 cells. Estrone and estrone sulfate also stimulated the proliferation of Ishikawa, showed a tendency to increase the proliferation of HEC-1-A and RL95-2 cells, but decreased the proliferation of KLE. Estrogens had no effect on the migration and clonogenicity of these four EC cell lines, however, there was a trend toward a smaller colony area for cells incubated with higher estrogen concentrations. We have previously shown that in EC estradiol forms from inactive estrone sulfate via the sulfatase pathway. This study showed that estrogens significantly promote the proliferation of grade 1 Ishikawa EC cells, and grade 2 RL95-2 and decrease the proliferation of grade 3 KLE cells. These differences in proliferation were associated with ERα positivity of Ishikawa cells and GPER expression in other cells. clonogenicity endometrial cancer estradiol estrone estrone-sulfate migration proliferation klonogenost rak endometrija estradiol estron estron-sulfat migracije širjenje true false true Angleški jezik Slovenski jezik Članek v reviji 2025-05-06 15:09:10 2025-05-06 15:09:14 2025-05-07 04:05:41 0000-00-00 00:00:00 2025 0 0 13 str. Vol. 251, art. 106762 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61:577.2 1879-1220 10.1016/j.jsbmb.2025.106762 234835459 1-s2.0-S0960076025000901-main.pdf 1-s2.0-S0960076025000901-main.pdf 1 A64E5050F8BB7E13C94A8FEB2BB6D9EE 53979ea6d39d5d125a6d5c19f84b64b9a9a2b85831791228b4bbf19704b2ab13 fceace50-2a7b-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210092 https://www.sciencedirect.com/science/article/pii/S0960076025000901 0 1f75a069-2a7a-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=210091 Medicinska fakulteta 0 0 0