Cancer is one of the leading causes of death worldwide, accounting for approximately 10 million deaths annually. In this master's thesis, we explored novel approaches to cancer treatment by focusing on voltage-gated proton channels Hv1, which are often overexpressed in various cancer cells. Hv1 channels play a role in regulating pH, cell proliferation, immune response, and the production of reactive oxygen species (ROS). Inhibition of these channels can reduce oxidative stress, suppress metastasis, and induce apoptosis in cancer cells. Based on the structure of the known compound NZ-49, we designed, synthesized, and analyzed five new derivatives featuring a 5-benzyloxyphenyl-2-aminoimidazole scaffold. The structure and purity of the compounds were confirmed using UHPLC, IR, NMR, and HRMS techniques. Biological evaluation was performed using the patch-clamp electrophysiological method on CHO cells expressing the human Hv1 channel. The results showed that most of the synthesized compounds inhibited Hv1 more effectively than the reference compound NZ-49, indicating their potential as therapeutic agents against cancer.
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