Beyond dimerization : harnessing tetrameric coiled-coils for nanostructure assembly

Vidmar, Sara (author), Šmidlehner, Tamara (author), Aupič, Jana (author), Strmšek, Žiga (author), Ljubetič, Ajasja (author), Xiao, Fei (author), Hu, Guang (author), Liu, Chuan (author), Beck, Florian (author), Erdmann, Philipp S. (author), Jerala, Roman (author)

Versatile DNA and polypeptide-based structures have been designed based on complementary modules. However, polypeptides can also form higher oligomeric states. We investigated the introduction of tetrameric modules as a substitute for coiled-coil dimerization units used in previous modular nanostructures. Tetramerizing helical bundles can run in parallel or antiparallel orientation, expanding the number of topological solutions for modular nanostructures. Furthermore, this strategy facilitates the construction of nanostructures from two identical polypeptide chains. Importantly, tetrameric modules substantially stabilized protein nanostructures against air–water interface denaturation, enabling the determination of the first cryo-electron microscopy three-dimensional structure of a coiled-coil-based nanostructure, confirming the designed agreement of the modules forming a tetrahedral cage.

• Language: English
• Keywords: coiled-coils, protein origami, cryo-electron, microscopy, protein design
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: 8 str.
• Numbering: Vol. 64, iss. 12, art. e202422075
• PID: 20.500.12556/RUL-167918
• UDC: 577
• ISSN on article: 1521-3773
• DOI: 10.1002/anie.202422075
• COBISS.SI-ID: 221895427
• Publication date in RUL: 20.03.2025

Record is a part of a journal

• Title: Angewandte Chemie : international edition
• Shortened title: Angew. Chem.
• Publisher: Wiley
• ISSN: 1521-3773
• COBISS.SI-ID: 21810181

Licences

• License: CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
• Link: http://creativecommons.org/licenses/by-nc/4.0/
• Description: A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

• Language: Slovenian
• Keywords: biokemija, DNK, polipeptidi, nanostrukture