20.500.12556/RUL-167918 Beyond dimerization harnessing tetrameric coiled-coils for nanostructure assembly Versatile DNA and polypeptide-based structures have been designed based on complementary modules. However, polypeptides can also form higher oligomeric states. We investigated the introduction of tetrameric modules as a substitute for coiled-coil dimerization units used in previous modular nanostructures. Tetramerizing helical bundles can run in parallel or antiparallel orientation, expanding the number of topological solutions for modular nanostructures. Furthermore, this strategy facilitates the construction of nanostructures from two identical polypeptide chains. Importantly, tetrameric modules substantially stabilized protein nanostructures against air–water interface denaturation, enabling the determination of the first cryo-electron microscopy three-dimensional structure of a coiled-coil-based nanostructure, confirming the designed agreement of the modules forming a tetrahedral cage. coiled-coils protein origami cryo-electron microscopy protein design biokemija DNK polipeptidi nanostrukture true false true Angleški jezik Slovenski jezik Članek v reviji 2025-03-20 10:27:51 2025-03-20 10:27:52 2025-03-21 03:57:50 0000-00-00 00:00:00 2025 0 0 8 str. iss. 12, art. e202422075 Vol. 64 Mar. 17, 2025 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 1521-3773 10.1002/anie.202422075 221895427 Angew_Chem_Int_Ed_-_2024_-_Vidmar_-_Beyond_Dimerization__Harnessing_Tetrameric_Coiled‐Coils_for_Nanostructure_Assembly.pdf Angew_Chem_Int_Ed_-_2024_-_Vidmar_-_Beyond_Dimerization__Harnessing_Tetrameric_Coiled‐Coils_for_Nanostructure_Assembly.pdf 1 419D979F117DDF0F6C4D4F4E16AF6D4D 6c0eecf78fcb72ac027764b657d0e53a7c139e92dfbcd0413c9b516c4743ae43 b1e0ef97-056d-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200836 https://onlinelibrary.wiley.com/doi/10.1002/anie.202422075 1 95f10c2c-056c-11f0-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=200834 Medicinska fakulteta 0 0 0