Titanium dioxide substrates as sensors for detection of platelets and extracellular particles from blood plasma

Rawat, Niharika (author), Junkar, Ita (author), Benčina, Metka (author), Lampe, Tomaž (author), Kralj-Iglič, Veronika (author), Iglič, Aleš (author)

Biosensors are pivotal in biomedical applications, particularly for disease detection, diagnosis, treatment, health management, and monitoring. Titanium dioxide (TiO2) is a prominent material for biosensors due to its biocompatibility, corrosion resistance, and availability in various nanostructured forms. This study explores the interaction of platelets and extracellular vesicles (EVs) with different TiO2 surface morphologies using flow cytometry (FCM) and scanning electron microscopy (SEM). Blood plasma samples were incubated with various TiO2 surfaces to evaluate particle counts and characteristics. FCM results indicated a higher abundance of platelets compared to EVs, with significant fragmentation observed post-centrifugation. SEM analysis confirmed platelet activation and fragmentation, with the microflowered TiO2 surface displaying fewer vesicles due to its rough topography. The findings suggest that while TiO2 surface structuring minimally impacts particle counts, it influences platelet and EV interactions, highlighting the need for advanced detection techniques and further investigation into surface interactions.

• Language: English
• Keywords: cold gaseous plasma, atmospheric pressure plasma, plasma technology, dental medicine, extracellular vesicles, surface treatment
• Work type: Other
• Typology: 1.08 - Published Scientific Conference Contribution
• Organization: ZF - Faculty of Health Sciences; FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 92-101
• PID: 20.500.12556/RUL-163381
• UDC: 577:616.15
• DOI: 10.55295/PSL.11.2024.11
• COBISS.SI-ID: 210339587
• Publication date in RUL: 04.10.2024

Record is a part of a monograph

• Title: Socratic Lectures : 11th international symposium, Ljubljana, 8. June 2024
• Editors: Veronika Kralj-Iglič, Yelena Istileulova, Anna Romolo
• Place of publishing: Ljubljana
• Publisher: University of Ljubljana Press
• Year: 2024
• ISBN: 978-961-297-383-4
• COBISS.SI-ID: 205044227

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.