Assessment of extracellular particles directly in plasma

Korenjak, Boštjan (author), Erjavec, Vladimira (author), Kralj-Iglič, Veronika (author)

Extracellular nanoparticles (EPs) in blood have been amply studied since their biological role was indicated more than 50 years ago. Among the various types of nano-sized blood constituents, the studies outlined lipoproteins, immune complexes and extracellular particles. Efforts have been invested to separate or separately assess different types of particles. Recently, much attention has been devoted to extracellular vesicles (EVs), however, experimental evidence indicates that the methods used for their harvesting and assessment importantly influence the content of the samples whereas the information on characteristics of the samples that could reflect the clinical status of the sample donor may be altered. Recent developments of the characterization methods point to the possibility of assessing nano-sized EPs directly in plasma and blood, thereby avoiding artefacts caused by processing. In this contribution, we consider the assessment of nano-sized EPs from blood and provide evidence supporting the direct assessment of EPs in plasma.

• Language: English
• Keywords: extracellular particles from blood, extracellular particles from plasma, interferometric light microscopy, dynamic light scattering
• Work type: Other
• Typology: 1.08 - Published Scientific Conference Contribution
• Organization: ZF - Faculty of Health Sciences; VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 71-77
• PID: 20.500.12556/RUL-163378
• UDC: 577
• DOI: 10.55295/PSL.11.2024.8
• COBISS.SI-ID: 210329603
• Publication date in RUL: 04.10.2024

Record is a part of a monograph

• Title: Socratic Lectures : 11th international symposium, Ljubljana, 8. June 2024
• Editors: Veronika Kralj-Iglič, Yelena Istileulova, Anna Romolo
• Place of publishing: Ljubljana
• Publisher: University of Ljubljana Press
• Year: 2024
• ISBN: 978-961-297-383-4
• COBISS.SI-ID: 205044227

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: J3-3066-2021
• Name: Optimizacija s trombociti in zunajceličnimi vezikli bogate avtologne krvne plazme za zdravljenje pooperativnih ran v otorinolaringologiji

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P3-0388-2022
• Name: Mehanizmi varovanja zdravja