Standard operating procedure for one - spin individualized therapeutic plasma based on a mathematical model and test spin

Kralj-Iglič, Veronika (author), Berry, Maxence (author), Božič, Darja (author), Romolo, Anna (author), Troha, Kaja (author), Arko, Matevž (author), Vozel, Domen (author), Iglič, Aleš (author), Battelino, Saba (author), Liguori, Giovanna L. (author), Kisslinger, Annamaria (author)

Standard operating procedure (SOP) for one-spin centrifugation of blood to prepare plasma is presented. The aim of the procedure is to prepare plasma with highest content of platelets and extracellular vesicles (EVs) from an individual blood sample. As sedimenting erythrocytes push plasma that carries platelets and EVs in the opposite direction, the optimal time is defined at the point when the lower bound of the ascending plasma meets the upper bound of the sedimenting erythrocytes. This process is described by a mathematical model which has one adjustable parameter. The parameter is estimated by a test spin at low centripetal acceleration of the centrifuge rotor and short centrifugation time. By using the mathematical model and the estimated parameter, the optimal time of centrifugation at chosen centripetal acceleration of the centrifuge rotor can be set. In the presented contribution we describe the protocole and state the elements needed for safe preparation of optimized and individualized plasma intended for regeneration.

• Language: English
• Keywords: therapeutic plasma, platelet rich plasma, platelet and extracellular vesicles rich plasma, regeneration, extracellular vesicles, extracellular particles, exosomes, sedimentation of erythrocytes
• Work type: Other
• Typology: 1.08 - Published Scientific Conference Contribution
• Organization: ZF - Faculty of Health Sciences; FE - Faculty of Electrical Engineering; MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 54-64
• PID: 20.500.12556/RUL-163188
• UDC: 577:616.15
• DOI: 10.55295/PSL.11.2024.6
• COBISS.SI-ID: 206514947
• Publication date in RUL: 03.10.2024

Record is a part of a monograph

• Title: Socratic Lectures : 11th international symposium, Ljubljana, 8. June 2024
• Editors: Veronika Kralj-Iglič, Yelena Istileulova, Anna Romolo
• Place of publishing: Ljubljana
• Publisher: University of Ljubljana Press
• Year: 2024
• ISBN: 978-961-297-383-4
• COBISS.SI-ID: 205044227

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.