20.500.12556/RUL-163188 Standard operating procedure for one - spin individualized therapeutic plasma based on a mathematical model and test spin Standard operating procedure (SOP) for one-spin centrifugation of blood to prepare plasma is presented. The aim of the procedure is to prepare plasma with highest content of platelets and extracellular vesicles (EVs) from an individual blood sample. As sedimenting erythrocytes push plasma that carries platelets and EVs in the opposite direction, the optimal time is defined at the point when the lower bound of the ascending plasma meets the upper bound of the sedimenting erythrocytes. This process is described by a mathematical model which has one adjustable parameter. The parameter is estimated by a test spin at low centripetal acceleration of the centrifuge rotor and short centrifugation time. By using the mathematical model and the estimated parameter, the optimal time of centrifugation at chosen centripetal acceleration of the centrifuge rotor can be set. In the presented contribution we describe the protocole and state the elements needed for safe preparation of optimized and individualized plasma intended for regeneration. therapeutic plasma platelet rich plasma platelet and extracellular vesicles rich plasma regeneration extracellular vesicles extracellular particles exosomes sedimentation of erythrocytes true false true Angleški jezik Ni določen Drugo 2024-10-03 14:20:34 2024-10-03 14:20:35 2024-10-04 04:15:58 0000-00-00 00:00:00 2024 0 0 Str. 54-64 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577:616.15 10.55295/PSL.11.2024.6 206514947 205044227 RAZ_Kralj-Iglic_Veronika_2024.pdf RAZ_Kralj-Iglic_Veronika_2024.pdf 1 C1868B8D7D1D41C866F8387CE5490D3E 2de79528554b7ccb5234449c7224da7f628484e405b96c7a103ca4abc56a2e9d bc32d8eb-8181-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=192724 https://www.zf.uni-lj.si/images/zalozba/Sokratska_11/6_Kralj-Igli%C4%8D_Veronika.pdf 0 f4fe0036-8181-11ef-b232-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=192725 Zdravstvena fakulteta Fakulteta za elektrotehniko Medicinska fakulteta 0 0 0