Vpliv substance P na izražanje katepsina S in CD74 v makrofagih in monocitih

Bohorč, Leila (author), Turk, Boris (mentor)

Katepsin S je cisteinska proteaza, ki sodeluje pri razvoju številnih bolezenskih stanj, znan pa je tudi po tem, da sodeluje pri razgradnji CD74 v endosomih. CD74 kot šaperon sodeluje pri izgradnji molekul MHC razreda II. Kot receptor v plazmalemi predstavlja vezavno mesto za MIF, ki sproži NF-κB signalno pot ter različne kinazne kaskade (ERK, PI3K-Akt in AMPK) in tako sodeluje pri vnetnih procesih. Katepsin S in CD74 se med drugim izražata v makrofagih in monocitih. Diplomsko delo primerja izražanje katepsina S in CD74 v makrofagih M0 in monocitih THP-1, pri čemer nas je zanimal tudi vpliv substance P. Katepsin S se izraža močneje kot CD74. Pokazali smo, da se proteina močneje izražata v monocitih, kjer substanca P ni imela nobenega vpliva. V makrofagih se z daljšim časom izpostavljenosti substanci P v obeh primerih poveča količina izraženih proteinov. Ti rezultati predstavljajo osnovo za nadaljnje analize in preučevanje izražanja katepsina S in CD74 ter vpliva substance P.

• Language: Slovenian
• Keywords: katepsin S, CD74, makrofagi, monociti, substanca P
• Work type: Bachelor thesis/paper
• Typology: 2.11 - Undergraduate Thesis
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2024
• PID: 20.500.12556/RUL-160410
• COBISS.SI-ID: 206067715
• Publication date in RUL: 28.08.2024

Secondary language

• Language: English
• Title: The effect of substance P on the expression of cathepsin S and CD74 in macrophages and monocytes

Abstract

Cathepsin S is a cysteine protease responsible for many pathological conditions and diseases, including cancer. It is also known as one of the proteases capable of cleaving CD74 in endosomes. CD74 is a chaperone participating in MHC class II assembly. As a receptor in plasma membrane, it functions as a binding site for MIF, with different signalling pathways, including NF-κB and kinase cascades, such as ERK, PI3K-Akt, and AMPK, which play a role in inflammation. Cathepsin S and CD74 are expressed in numerous types of cells, including macrophages and monocytes. This thesis compares the expression of cahtepsin S and CD74 in macrophages M0 and monocytes THP-1 and describes the effect of substance P. The expression of cathepsin S is higher than that of CD74. The expression levels of both proteins were higher in monocytes, with substance P having no effect. In macrophages, the expression increased with prolonged treatment of cells with substance P. These results provide the basis for future analysis of cathepsin S and CD74 expression, as well as the effects of substance P.

• Keywords: cathepsin S, CD74, macrophages, monocytes, substance P