20.500.12556/RUL-160410 Vpliv substance P na izražanje katepsina S in CD74 v makrofagih in monocitih The effect of substance P on the expression of cathepsin S and CD74 in macrophages and monocytes Katepsin S je cisteinska proteaza, ki sodeluje pri razvoju številnih bolezenskih stanj, znan pa je tudi po tem, da sodeluje pri razgradnji CD74 v endosomih. CD74 kot šaperon sodeluje pri izgradnji molekul MHC razreda II. Kot receptor v plazmalemi predstavlja vezavno mesto za MIF, ki sproži NF-κB signalno pot ter različne kinazne kaskade (ERK, PI3K-Akt in AMPK) in tako sodeluje pri vnetnih procesih. Katepsin S in CD74 se med drugim izražata v makrofagih in monocitih. Diplomsko delo primerja izražanje katepsina S in CD74 v makrofagih M0 in monocitih THP-1, pri čemer nas je zanimal tudi vpliv substance P. Katepsin S se izraža močneje kot CD74. Pokazali smo, da se proteina močneje izražata v monocitih, kjer substanca P ni imela nobenega vpliva. V makrofagih se z daljšim časom izpostavljenosti substanci P v obeh primerih poveča količina izraženih proteinov. Ti rezultati predstavljajo osnovo za nadaljnje analize in preučevanje izražanja katepsina S in CD74 ter vpliva substance P. Cathepsin S is a cysteine protease responsible for many pathological conditions and diseases, including cancer. It is also known as one of the proteases capable of cleaving CD74 in endosomes. CD74 is a chaperone participating in MHC class II assembly. As a receptor in plasma membrane, it functions as a binding site for MIF, with different signalling pathways, including NF-κB and kinase cascades, such as ERK, PI3K-Akt, and AMPK, which play a role in inflammation. Cathepsin S and CD74 are expressed in numerous types of cells, including macrophages and monocytes. This thesis compares the expression of cahtepsin S and CD74 in macrophages M0 and monocytes THP-1 and describes the effect of substance P. The expression of cathepsin S is higher than that of CD74. The expression levels of both proteins were higher in monocytes, with substance P having no effect. In macrophages, the expression increased with prolonged treatment of cells with substance P. These results provide the basis for future analysis of cathepsin S and CD74 expression, as well as the effects of substance P. katepsin S CD74 makrofagi monociti substanca P cathepsin S CD74 macrophages monocytes substance P true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2024-08-28 09:55:00 2024-08-28 09:55:03 2024-09-02 11:49:41 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 24130 206067715 Bohorc_Leila_-_Vpliv_substance_P_na_izrazanje_katepsina_S_in_CD74_v_makrofagih_in_monocitih.pdf Bohorc_Leila_-_Vpliv_substance_P_na_izrazanje_katepsina_S_in_CD74_v_makrofagih_in_monocitih.pdf 1 8350AB935F13E0C6F6AA37CDFBC920B2 9c2716e8aad2e769115fc909fe1042ff11b69ba76ceddd6338df57b0b80e5702 9bb7e988-6512-11ef-8f74-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=189095 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0