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Searching for alternative high DNA-yielding bone types for DNA analysis of aged skeletal remains
ID
Golob, Aja
(
Avtor
),
ID
Kravanja, Pia
(
Avtor
),
ID
Concato, Monica
(
Avtor
),
ID
Leskovar, Tamara
(
Avtor
),
ID
Zupanič-Pajnič, Irena
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,43 MB)
MD5: EFE990E262B3827EBAD4B2D10906E053
URL - Izvorni URL, za dostop obiščite
https://www.sciencedirect.com/science/article/pii/S0379073824002652
Galerija slik
Izvleček
The petrous bone contains significantly higher amounts of DNA than any other human bone. Because of highly destructive sampling and because it is not always part of the recovered remains, the need for alternative sources of DNA is important. To identify additional optimal bone types, petrous bones were compared to femurs, tali, and calcanei sampled from 66 adult skeletons from two distinct modern-era Christian cemeteries. An extraction method employing full demineralization was used to obtain DNA, real-time PCR quantification to ascertain DNA quantity and degradation, and a commercial forensic short tandem repeats (STR) PCR amplification kit to determine genetic profiles. Statistical analysis was performed to explore the differences in DNA yield, DNA degradation, and success of STR amplification. A systematic studies exploring intra-skeletal variability in DNA preservation including various excavation sites differing by time period and geographical position are rare, and the second part of the investigation was based on a comparison of both archaeological sites, which allowed us to compare the effect of different post-mortem intervals and environmental conditions on DNA preservation. The older burial site in Črnomelj was active between the 13th and 18th century, whereas the more recent Polje burial was in use from the 16th to 19th century, creating different temporal and geographical environments. Results for the Črnomelj burial site revealed that the petrous bone outperformed all other bone types studied, except the calcaneus. At the Polje archeological site calcanei, tali, and femurs yielded the same STR typing success as petrous bones. The results obtained highlight the importance of careful bone sample selection for DNA analysis of aged skeletal remains. In addition to petrous bones, calcanei were found to be an alternative source of DNA when older burial sites are investigated. When more recent burial sites are processed, calcanei, tali, and femurs should be sampled besides petrous bones, not only because they exhibited good performance, but also because of easier sampling and easier grinding in the case of trabecular bones. This study contributes valuable insights into the potential use of various skeletal types as a source of DNA for investigation of aged skeletal remains, and it offers practical implications for forensic and archaeological investigations.
Jezik:
Angleški jezik
Ključne besede:
aged skeletal remains
,
ancient DNA
,
DNA preservation
,
sampling strategy
,
STR typing
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
MF - Medicinska fakulteta
FF - Filozofska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2024
Št. strani:
10 str.
Številčenje:
Vol. 362, art. 112184
PID:
20.500.12556/RUL-160101
UDK:
61
ISSN pri članku:
1872-6283
DOI:
10.1016/j.forsciint.2024.112184
COBISS.SI-ID:
203563779
Datum objave v RUL:
20.08.2024
Število ogledov:
81
Število prenosov:
4
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Objavi na:
Gradivo je del revije
Naslov:
Forensic science international
Založnik:
Elsevier
ISSN:
1872-6283
COBISS.SI-ID:
23017945
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
ostareli skeletni ostanki
,
starodavna DNK
,
ohranjanje DNA
,
strategija vzorčenja
,
STR tipizacija
Projekti
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
J3-3080
Naslov:
Določitev predniškega porekla iz DNA za identifikacijske namene
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