Postmortem chondrocyte viability in porcine articular cartilage : influence of time, temperature, and burial under winter conditions

Cvetko, Marko (author), Knific, Tanja (author), Frangež, Robert (author), Motaln, Helena (author), Rogelj, Boris (author), Alibegović, Armin (author), Gombač, Mitja (author)

The aim of the present study was to investigate the effects of time, temperature, and burial in a natural environment on the viability of chondrocytes in porcine femoral condyles using confocal laser scanning microscopy. Hind trotters from 10 pigs were buried or left unburied. Samples were collected daily and stained with a combination of vital dyes (calcein-AM and ethidium homodimer-1). The chondrocytes showed an intense staining corresponding to their vitality. In the first 3 days, viability decreased slowly and showed no statistical difference between buried and unburied samples. After the first 3 days, it decreased rapidly, with the viability of the buried samples being 66% on day 4, decreasing to 25% on day 8 and to 16% on day 10, while in the unburied samples it decreased to 43% on day 4, 13% on day 8 and 5% on day 10. Our results indicate a time, temperature, and burial dependent decrease in chondrocyte viability and suggest the use of chondrocyte viability as a marker for estimating PMI in both the natural environment and in animals, as well as its potential use in humans.

• Language: English
• Keywords: burial, calcein-AM, cartilage, confocal laser scanning microscope, CLSM, ethidium homodimer-1, postmortem interval, PMI, swine
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 1094-1101
• Numbering: Vol. 69, iss. 3
• PID: 20.500.12556/RUL-159214
• UDC: 636.09:616
• ISSN on article: 1556-4029
• DOI: 10.1111/1556-4029.15503
• COBISS.SI-ID: 189439235
• Publication date in RUL: 03.07.2024

Record is a part of a journal

• Title: Journal of forensic sciences
• Publisher: Wiley, American Academy of Forensic Sciences
• ISSN: 1556-4029
• COBISS.SI-ID: 515028249

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: prašiči, hondrociti, sklepni hrustanec, postmortalna sposobnost preživetja

Projects

• Funder: ARRS - Slovenian Research Agency
• Funding programme: Young researchers

• Funder: ARRS - Slovenian Research Agency
• Project number: P4-0092
• Name: Zdravje živali, okolje in varna hrana