Postmortem chondrocyte viability in porcine articular cartilage : influence of time, temperature, and burial under winter conditions

Cvetko, Marko (avtor), Knific, Tanja (avtor), Frangež, Robert (avtor), Motaln, Helena (avtor), Rogelj, Boris (avtor), Alibegović, Armin (avtor), Gombač, Mitja (avtor)

The aim of the present study was to investigate the effects of time, temperature, and burial in a natural environment on the viability of chondrocytes in porcine femoral condyles using confocal laser scanning microscopy. Hind trotters from 10 pigs were buried or left unburied. Samples were collected daily and stained with a combination of vital dyes (calcein-AM and ethidium homodimer-1). The chondrocytes showed an intense staining corresponding to their vitality. In the first 3 days, viability decreased slowly and showed no statistical difference between buried and unburied samples. After the first 3 days, it decreased rapidly, with the viability of the buried samples being 66% on day 4, decreasing to 25% on day 8 and to 16% on day 10, while in the unburied samples it decreased to 43% on day 4, 13% on day 8 and 5% on day 10. Our results indicate a time, temperature, and burial dependent decrease in chondrocyte viability and suggest the use of chondrocyte viability as a marker for estimating PMI in both the natural environment and in animals, as well as its potential use in humans.

• Jezik: Angleški jezik
• Ključne besede: burial, calcein-AM, cartilage, confocal laser scanning microscope, CLSM, ethidium homodimer-1, postmortem interval, PMI, swine
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 1094-1101
• Številčenje: Vol. 69, iss. 3
• PID: 20.500.12556/RUL-159214
• UDK: 636.09:616
• ISSN pri članku: 1556-4029
• DOI: 10.1111/1556-4029.15503
• COBISS.SI-ID: 189439235
• Datum objave v RUL: 03.07.2024

Gradivo je del revije

• Naslov: Journal of forensic sciences
• Založnik: Wiley, American Academy of Forensic Sciences
• ISSN: 1556-4029
• COBISS.SI-ID: 515028249

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: prašiči, hondrociti, sklepni hrustanec, postmortalna sposobnost preživetja

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Program financ.: Young researchers

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P4-0092
• Naslov: Zdravje živali, okolje in varna hrana