Platelet activation and blood extracellular vesicles : the influence of venepuncture and short blood storage

Marić, Ivica (author), Žiberna, Klemen (author), Kolenc, Ana (author), Maličev, Elvira (author)

Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating EVs. Material: EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs. Results: In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype. Conclusions: Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known.

• Language: English
• Keywords: blood, blood plasma, extracellular vesicles, flow cytometry, platelet activation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine; BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 7 str.
• Numbering: Vol. 106, art. 102842
• PID: 20.500.12556/RUL-156259
• UDC: 616.1
• ISSN on article: 1079-9796
• DOI: 10.1016/j.bcmd.2024.102842
• COBISS.SI-ID: 192381699
• Publication date in RUL: 16.05.2024

Record is a part of a journal

• Title: Blood cells, molecules & diseases
• Shortened title: Blood cells mol. diseases
• Publisher: Elsevier
• ISSN: 1079-9796
• COBISS.SI-ID: 1450773

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: kri, krvna plazma, zunajcelični vezikli, pretočna citometrija, aktivacija trombocitov