Platelet activation and blood extracellular vesicles : the influence of venepuncture and short blood storage

Marić, Ivica (avtor), Žiberna, Klemen (avtor), Kolenc, Ana (avtor), Maličev, Elvira (avtor)

Extracellular vesicles (EVs) as membrane-bound particles released by various cells are potential tools for diagnosis and treatment. Blood cells, particularly platelets, are the source of circulating EVs. Material: EVs were enriched with gradient ultracentrifugation and measured by nanoparticle tracking assay. A flow cytometric multiplex assay was used for cellular source determination. Activation of platelets was measured as a percentage of CD62p+/CD61+ platelets and correlated with the concentration and size of released EVs. Results: In general there was no statistically significant correlation between EVs` concentration and degree of platelet activation. EVs from different cellular sources were detected. Comparing different needle thicknesses, there was a decrease in the EVs concentration for the 16G needle versus the 21G needle, but no difference was observed for EVs` size and phenotype or platelets activation. During blood storage, platelet activation increased, but there was no effect on the EVs` concentration, size, or phenotype. Conclusions: Preanalytical factors like needle thickness and storage time can affect the MVs' properties. Activation of platelets during blood collection or blood storage occurs; however, it is difficult to determine its effect on the physiological properties of EVs since the mechanisms of EVs` biogenesis and especially clearness are not precisely known.

• Jezik: Angleški jezik
• Ključne besede: blood, blood plasma, extracellular vesicles, flow cytometry, platelet activation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 7 str.
• Številčenje: Vol. 106, art. 102842
• PID: 20.500.12556/RUL-156259
• UDK: 616.1
• ISSN pri članku: 1079-9796
• DOI: 10.1016/j.bcmd.2024.102842
• COBISS.SI-ID: 192381699
• Datum objave v RUL: 16.05.2024

Gradivo je del revije

• Naslov: Blood cells, molecules & diseases
• Skrajšan naslov: Blood cells mol. diseases
• Založnik: Elsevier
• ISSN: 1079-9796
• COBISS.SI-ID: 1450773

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: kri, krvna plazma, zunajcelični vezikli, pretočna citometrija, aktivacija trombocitov