Experimental and theoretical model for the origin of coiling of cellular protrusions around fibers

Kumar Sadhu, Raj (author), Hernandez-Padilla, Christian (author), Eshed Eisenbach, Yael (author), Penič, Samo (author), Zhang, Lixia (author), Vishwasrao, Harshad D. (author), Behkam, Bahareh (author), Konstantopoulos, Konstantinos (author), Shroff, Hari (author), Iglič, Aleš (author), Peles, Elior (author), Nain, Amrinder S. (author), Gov, Nir S. (author)

Protrusions at the leading-edge of a cell play an important role in sensing the extracellular cues during cellular spreading and motility. Recent studies provided indications that these protrusions wrap (coil) around the extracellular fibers. However, the physics of this coiling process, and the mechanisms that drive it, are not well understood. We present a combined theoretical and experimental study of the coiling of cellular protrusions on fibers of different geometry. Our theoretical model describes membrane protrusions that are produced by curved membrane proteins that recruit the protrusive forces of actin polymerization, and identifies the role of bending and adhesion energies in orienting the leading-edges of the protrusions along the azimuthal (coiling) direction. Our model predicts that the cell’s leading-edge coils on fibers with circular cross-section (above some critical radius), but the coiling ceases for flattened fibers of highly elliptical cross-section. These predictions are verified by 3D visualization and quantitation of coiling on suspended fibers using DualView light-sheet microscopy (diSPIM). Overall, we provide a theoretical framework, supported by experiments, which explains the physical origin of the coiling phenomenon.

• Language: English
• Keywords: cell motility, computer simulations, Monte Carlo method, physical protein model
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: Str. 1-13
• Numbering: Vol. 14, [article no.] 5612
• PID: 20.500.12556/RUL-155480
• UDC: 577
• ISSN on article: 2041-1723
• DOI: 10.1038/s41467-023-41273-y
• COBISS.SI-ID: 166115587
• Publication date in RUL: 04.04.2024

Record is a part of a journal

• Title: Nature communications
• Shortened title: Nat. commun.
• Publisher: Springer Nature
• ISSN: 2041-1723
• COBISS.SI-ID: 2315876

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: mobilnost celice, računalniške simulacije, metoda Monte Carlo, fizikalni model proteinov