20.500.12556/RUL-155480 Experimental and theoretical model for the origin of coiling of cellular protrusions around fibers Protrusions at the leading-edge of a cell play an important role in sensing the extracellular cues during cellular spreading and motility. Recent studies provided indications that these protrusions wrap (coil) around the extracellular fibers. However, the physics of this coiling process, and the mechanisms that drive it, are not well understood. We present a combined theoretical and experimental study of the coiling of cellular protrusions on fibers of different geometry. Our theoretical model describes membrane protrusions that are produced by curved membrane proteins that recruit the protrusive forces of actin polymerization, and identifies the role of bending and adhesion energies in orienting the leading-edges of the protrusions along the azimuthal (coiling) direction. Our model predicts that the cell’s leading-edge coils on fibers with circular cross-section (above some critical radius), but the coiling ceases for flattened fibers of highly elliptical cross-section. These predictions are verified by 3D visualization and quantitation of coiling on suspended fibers using DualView light-sheet microscopy (diSPIM). Overall, we provide a theoretical framework, supported by experiments, which explains the physical origin of the coiling phenomenon. cell motility computer simulations Monte Carlo method physical protein model mobilnost celice računalniške simulacije metoda Monte Carlo fizikalni model proteinov true false true Angleški jezik Slovenski jezik Članek v reviji 2024-04-04 10:25:07 2024-04-04 10:25:11 2024-04-05 03:46:23 0000-00-00 00:00:00 2023 0 0 Str. 1-13 Vol. 14, [article no.] 5612 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 577 2041-1723 10.1038/s41467-023-41273-y 166115587 s41467-023-41273-y_(1).pdf s41467-023-41273-y_(1).pdf 1 CE51CBE667B94427D4A22D39138C7D50 543db0c188ba596b0e571a79dee4e334da3b5be0499ce9369fb31ba6495f3519 fe388290-f25d-11ee-87dd-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182398 https://www.nature.com/articles/s41467-023-41273-y 1 dad8f845-f25c-11ee-87dd-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=182396 Fakulteta za elektrotehniko 0 0 0