The purpose of the master's thesis was to examine the antifungal and antimycotoxigenic
effects of encapsulated essential oil of winter savory (Satureja montana L.) on
filamentous fungi of three selected toxigenic species within the Aspergillus spp.
Microcarriers of winter savory essential oil in β-cyclodextrin were prepared at various
mass ratios (10:90, 20:80, 30:70). In an in vitro system, monitoring the concentration
of conidia of filamentous fungi (CFU/mL), measuring colony diameter on solid
Sabouraud maltose agar (SMA), calculating the inhibition percentage, and weighing
dry biomass revealed that the inhibitory effect of microcarriers on the growth of
filamentous fungi increased with the increasing mass ratio of essential oil and β
cyclodextrin. Microscopic examination of the cultures showed that microcarriers
allowed the growth of hyphae, but conidiophore heads were not observed. Using ultra
high-performance liquid chromatography (UHPLC) and a commercial competitive
enzyme-linked immunosorbent assay (ELISA) kit for the quantitative analysis of
ochratoxin A, we investigated the impact of microcarriers on the biosynthesis of
mycotoxins in liquid medium with yeast extract and sucrose (YES). As Aspergillus
ochraceus lost its toxigenicity, we couldn't verify the inhibitory effect on ochratoxin A
synthesis. However, with filamentous fungi Aspergillus flavus and Aspergillus
parasiticus, microcarriers prevented the synthesis of aflatoxin B1 , B2 , and G1. The
influence of microcarriers on the growth and mycotoxigenic activity of filamentous
fungi was also examined in a model food - tomato juice. With microcarriers at a mass
ratio of 20:80, we most effectively inhibited the growth and sporulation of filamentous
fungi in the model food between the 6th and 8th day of sample incubation. However,
the absence of mycotoxin synthesis in control samples prevented confirmation of the
impact of microcarriers on inhibiting mycotoxin synthesis.
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