20.500.12556/RUL-154752 Zaviranje rasti in mikotoksigene aktivnosti nitastih gliv treh izbranih vrst rodu Aspergillus s kapsuliranimi eteričnimi olji Inhibition of growth and mycotoxigenic activity of filamentous fungi of three selected species of the genus Aspergillus with encapsulated essential oils Namen magistrskega dela je bil preveriti protiglivno in protimikotoksigeno delovanje kapsuliranega eteričnega olja kraškega šetraja (Satureja montana L.) na nitaste glive treh izbranih vrst toksigenih nitastih gliv rodu Aspergillus spp. Mikrodelce eteričnega olja kraškega šetraja v β-ciklodektrinu smo pripravli v različnih masnih razmerjih (10:90, 20:80, 30:70). V in vitro sistemu smo s spremljanjem koncentracije konidijev nitastih gliv (CFU/mL), merjenjem premera kolonij na trdnem gojišču Sabouraud maltoza agar (SMA) in izračunom deleža inhibicije, kot tudi tehtanjem suhe biomase ugotovili, da se s povečanjem masnega razmerja eteričnega olja in β-ciklodekstrina poveča tudi zaviralni učinek mikrodelcev na rast nitastih gliv. Ob pregledu mikroskopskih kultur smo ugotovili, da mikrodelci omogočajo rast hif, vendar konidijskih glavic nismo zaznali. Z uporabo tekočinske kromatografije ultra visoke ločljivosti (UHPLC) in komercialnega kompleta kompetitivnega encimsko imunskega testa (ELISA) za kvantitativno analizo ohratoksina A smo preverili vpliv mikrodelcev na biosintezo mikotoksinov v tekočem gojišču s kvasnim ekstraktom in saharozo (YES). Ker je vrsta Aspergillus ochraceus izgubila toksigenost, nismo mogli preveriti zaviralnega učinka na sintezo ohratoksina A, medtem ko smo pri nitastih glivah Aspergillus flavus in Aspergillus parasiticus z uporabo mikrodelcev preprečili sintezo aflatoksina B1, B2 in G1. Vpliv mikrodelcev na rast in mikotoksigeno aktivnost nitastih gliv smo preverjali tudi v modelnem živilu - paradižnikovem soku. Z mikrodelci masnega razmerja 20:80 smo rast in sporulacijo nitastih gliv v modelnem živilu najuspešnejše zavrli med 6.-8. dnevom inkubacije vzorcev. Ker pa sinteze mikotoksinov nismo zaznali niti v kontrolnih vzorcih modelnega živila, nismo mogli potrditi vpliva mikrodelcev na zaviranje sinteze mikotoksinov. The purpose of the master's thesis was to examine the antifungal and antimycotoxigenic effects of encapsulated essential oil of winter savory (Satureja montana L.) on filamentous fungi of three selected toxigenic species within the Aspergillus spp. Microcarriers of winter savory essential oil in β-cyclodextrin were prepared at various mass ratios (10:90, 20:80, 30:70). In an in vitro system, monitoring the concentration of conidia of filamentous fungi (CFU/mL), measuring colony diameter on solid Sabouraud maltose agar (SMA), calculating the inhibition percentage, and weighing dry biomass revealed that the inhibitory effect of microcarriers on the growth of filamentous fungi increased with the increasing mass ratio of essential oil and β cyclodextrin. Microscopic examination of the cultures showed that microcarriers allowed the growth of hyphae, but conidiophore heads were not observed. Using ultra high-performance liquid chromatography (UHPLC) and a commercial competitive enzyme-linked immunosorbent assay (ELISA) kit for the quantitative analysis of ochratoxin A, we investigated the impact of microcarriers on the biosynthesis of mycotoxins in liquid medium with yeast extract and sucrose (YES). As Aspergillus ochraceus lost its toxigenicity, we couldn't verify the inhibitory effect on ochratoxin A synthesis. However, with filamentous fungi Aspergillus flavus and Aspergillus parasiticus, microcarriers prevented the synthesis of aflatoxin B1 , B2 , and G1. The influence of microcarriers on the growth and mycotoxigenic activity of filamentous fungi was also examined in a model food - tomato juice. With microcarriers at a mass ratio of 20:80, we most effectively inhibited the growth and sporulation of filamentous fungi in the model food between the 6th and 8th day of sample incubation. However, the absence of mycotoxin synthesis in control samples prevented confirmation of the impact of microcarriers on inhibiting mycotoxin synthesis. mikotoksigene nitaste glive  Aspergillus spp. mikotoksini [beta]-ciklodekstrin eterična olja kraški šetraj Satureja montana protimikrobne snovi mycotoxigenic filamentous fungi aspergillus spp. mycotoxins [beta]-cyclodextrin essential oils karst savory Satureja montana antimicrobial substances true false false [M. Bračun] Slovenski jezik Angleški jezik Magistrsko delo/naloga 2024-02-29 07:15:21 2024-02-29 07:15:33 2024-03-04 10:27:41 0000-00-00 00:00:00 2024 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 579.24:582.123.4:615.9:547.913 236300 187516419 38502.pdf 38502.pdf 1 E9BC6293D0E29087F423328F7E174EC7 1523b550b1d8b96ed313ed232244f963ad6bc64424501c8aa5c641f8dfafe033 fef40bf2-d6c9-11ee-ac9d-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=181316 Biotehniška fakulteta 0 0 0