Effect of sucrose concentration on streptococcus mutans adhesion to dental material surfaces

Zore, Anamarija (author), Rojko, Franc (author), Matijaković Mlinarić, Nives (author), Veber, Jona (author), Učakar, Aleksander (author), Štukelj, Roman (author), Pondelak, Andreja (author), Sever Škapin, Andrijana (author), Bohinc, Klemen (author)

Enamel demineralization, known as dental caries, is instigated by the bacterium Streptococcus mutans, which generates acid during carbohydrate metabolism. Among carbohydrates, sucrose is the most cariogenic and capable of biofilm formation. This study aimed to explore and comprehend Streptococcus mutans’ adherence to two prevalent dental material surfaces, i.e., a cobalt–chromium (Co-Cr) alloy and a resin-based composite, under the influence of various sucrose concentrations. To understand bacterial adhesion, the surfaces were characterized using profilometry, tensiometry, and surface charge measurements. Bacterial adhesion was evaluated using scanning electron microscopy and crystal violet dye methods. Results revealed that the composite surface exhibited greater roughness compared with the Co-Cr alloy surface. Both surfaces displayed hydrophilic properties and a negative surface charge. Bacterial adhesion experiments indicated lower bacterial adherence to the Co-Cr alloy than to the composite surface before the addition of sucrose. However, the introduction of sucrose resulted in biofilm development on both surfaces, showcasing a similar increase in bacterial adhesion, with the highest levels being observed at a 5% sucrose concentration in the bacterial suspension. In conclusion, the findings suggest sucrose-rich foods could facilitate bacterial adaptation despite less favourable surface characteristics, thereby promoting biofilm formation.

• Language: English
• Keywords: bacterial adhesion, streptococcus mutans, dental material surfaces, sucrose concentration
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: 14 str.
• Numbering: Vol. 14, iss. 2, art. 165
• PID: 20.500.12556/RUL-154224-a0f0b533-a947-a50b-47bd-b16bfc5fe5eb
• UDC: 616.31
• ISSN on article: 2079-6412
• DOI: 10.3390/coatings14020165
• COBISS.SI-ID: 182777603
• Publication date in RUL: 02.02.2024

Record is a part of a journal

• Title: Coatings
• Shortened title: Coatings
• Publisher: MDPI AG
• ISSN: 2079-6412
• COBISS.SI-ID: 523035673

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 27.01.2024

Secondary language

• Language: Slovenian
• Keywords: adhezija bakterij, streptococcus mutans, površine dentalnih materialov, površine dentalnih materialov