20.500.12556/RUL-154224 Effect of sucrose concentration on streptococcus mutans adhesion to dental material surfaces Enamel demineralization, known as dental caries, is instigated by the bacterium Streptococcus mutans, which generates acid during carbohydrate metabolism. Among carbohydrates, sucrose is the most cariogenic and capable of biofilm formation. This study aimed to explore and comprehend Streptococcus mutans’ adherence to two prevalent dental material surfaces, i.e., a cobalt–chromium (Co-Cr) alloy and a resin-based composite, under the influence of various sucrose concentrations. To understand bacterial adhesion, the surfaces were characterized using profilometry, tensiometry, and surface charge measurements. Bacterial adhesion was evaluated using scanning electron microscopy and crystal violet dye methods. Results revealed that the composite surface exhibited greater roughness compared with the Co-Cr alloy surface. Both surfaces displayed hydrophilic properties and a negative surface charge. Bacterial adhesion experiments indicated lower bacterial adherence to the Co-Cr alloy than to the composite surface before the addition of sucrose. However, the introduction of sucrose resulted in biofilm development on both surfaces, showcasing a similar increase in bacterial adhesion, with the highest levels being observed at a 5% sucrose concentration in the bacterial suspension. In conclusion, the findings suggest sucrose-rich foods could facilitate bacterial adaptation despite less favourable surface characteristics, thereby promoting biofilm formation. bacterial adhesion streptococcus mutans dental material surfaces sucrose concentration adhezija bakterij streptococcus mutans površine dentalnih materialov površine dentalnih materialov true false true Angleški jezik Slovenski jezik Članek v reviji 2024-02-02 09:39:48 2024-02-02 09:39:49 2025-08-08 13:30:33 0000-00-00 00:00:00 2024 0 0 14 str. iss. 2, art. 165 Vol. 14 2024 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 616.31 2079-6412 10.3390/coatings14020165 182777603 zore24-coatings.pdf zore24-coatings.pdf 1 8C64A3F136806DF5A3A8AFCD3D6C5569 1e8616aaad6a414609997aaebe8ac1d2e8aee2718b1e116eaf7038b370dbd4a8 f42394ac-c1a6-11ee-9ac8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180492 https://www.mdpi.com/2079-6412/14/2/165 1 a15b40b9-c1a6-11ee-9ac8-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=180491 Zdravstvena fakulteta 0 0 0