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Dendritic cell activation and cytokine response in vaccine breakthrough TBE patients after in vitro stimulation with TBEV
ID
Marušić, Miša
(
Author
),
ID
Kopitar, Andreja Nataša
(
Author
),
ID
Korva, Miša
(
Author
),
ID
Knap, Nataša
(
Author
),
ID
Bogovič, Petra
(
Author
),
ID
Strle, Franc
(
Author
),
ID
Ihan, Alojz
(
Author
),
ID
Avšič-Županc, Tatjana
(
Author
)
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https://www.frontiersin.org/articles/10.3389/fimmu.2023.1190803/full
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Abstract
Tick-borne encephalitis (TBE) is a viral infection of the human central nervous system caused by the TBE virus (TBEV). The most effective protective measure against TBE is vaccination. Despite the highly immunogenic vaccine, cases of vaccine breakthroughs (VBTs) occur. One of the first targets of infection is dendritic cells (DC), which represent a fundamental bridge between innate and adaptive immunity through antigen presentation, costimulation, and cytokine production. Therefore, we investigated the activation and maturation of DCs and cytokine production after in vitro TBEV stimulation of peripheral blood mononuclear cells (PBMCs) obtained from VBT and unvaccinated TBE patients. Our results showed that the expression of HLA-DR and CD86 on DCs, was upregulated to a similar extent in both vaccinated and unvaccinated TBE patients but differed in cytokine production after stimulation with TBEV. PBMCs from patients with VBT TBE responded with lower levels of IFN-α and the proinflammatory cytokines IL-12 (p70) and IL-15 after 24- and 48-hour in vitro stimulation with TBEV, possibly facilitating viral replication and influencing the development of cell-mediated immunity. On the other hand, significantly higher levels of IL-6 in addition to an observed trend of higher expression of TNF-α measured after 6 days of in vitro stimulation of PBMC could support disruption of the blood–brain barrier and promote viral and immune cell influx into the CNS, leading to more severe disease in VBT TBE patients.
Language:
English
Keywords:
tick-borne encephalitis
,
vaccine breakthrough
,
cytokines
,
dendritic cells
,
immune response in vitro
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2023
Number of pages:
9 str.
Numbering:
Vol. 14, art. 1190803
PID:
20.500.12556/RUL-153997
UDC:
616.9
ISSN on article:
1664-3224
DOI:
10.3389/fimmu.2023.1190803
COBISS.SI-ID:
152562691
Publication date in RUL:
18.01.2024
Views:
476
Downloads:
34
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Record is a part of a journal
Title:
Frontiers in immunology
Shortened title:
Front. immunol.
Publisher:
Frontiers Media
ISSN:
1664-3224
COBISS.SI-ID:
30774233
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
klopni encefalitis
,
preboj cepiva
,
citokini
Projects
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
P3-0083
Name:
Odnosi parazitskega obstajanja
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
P3-0296
Name:
Bolezni in povzročitelji, ki jih v Sloveniji prenašajo členonožci
Funder:
EC - European Commission
Funding programme:
H2020
Project number:
871029
Name:
European Virus Archive GLOBAL
Acronym:
EVA-GLOBAL
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
I0-0022
Name:
Mreža raziskovalnih infrastrukturnih centrov Univerze v Ljubljani (MRIC UL)
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