QAC resistance genes in ESBL-producing E. coli isolated from patients with lower respiratory tract infections in the Central Slovenia region—a 21-year survey

Hrovat, Katja (avtor), Čremožnik Zupančič, Jerneja (avtor), Seme, Katja (avtor), Ambrožič, Jerneja (avtor)

Biocidal products prevent the spread of pathogenic microorganisms, including extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC), which is one of the most alarming health problems worldwide. Quaternary ammonium compounds (QACs) are surface-active agents that interact with the cytoplasmic membrane and are widely used in hospitals and food processing environments. A collection of 577 ESBL-EC, isolated from lower respiratory tract (LRT) samples, was screened for QAC resistance genes oqxA; oqxB; qacE∆1; qacE; qacF/H/I; qacG; sugE (p); emrE; mdfA; sugE (c); ydgE; ydgF; and for class 1, 2, and 3 integrons. The prevalence of chromosome-encoded genes ranged from 77 to 100%, while the prevalence of QAC resistance genes encoded on mobile genetic elements (MGEs) was relatively low (0–0.9%), with the exception of qacE∆1 (54.6%). PCR screening detected the presence of class 1 integrons in 36.3% (n = 210) of isolates, which were positively correlated with qacE∆1. More correlations between QAC resistance genes, integrons, sequence type group ST131, and β-lactamase genes were presented. The results of our study confirm the presence of QAC resistance genes and also class 1 integrons commonly found in multidrug-resistant clinical isolates and highlight the potential role of QAC resistance genes in the selection of ESBL-producing E. coli in hospitals.

• Jezik: Angleški jezik
• Ključne besede: Escherichia coli, lower respiratory tract, extended-spectrum β-lactamases, resistance gene, QAC, lower respiratory tract samples
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta ; MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 12 str.
• Številčenje: Vol. 8, iss. 5, art. 273
• PID: 20.500.12556/RUL-153248
• UDK: 579
• ISSN pri članku: 2414-6366
• DOI: 10.3390/tropicalmed8050273
• COBISS.SI-ID: 152196611
• Datum objave v RUL: 20.12.2023

Gradivo je del revije

• Naslov: Tropical medicine and infectious disease
• Skrajšan naslov: Trop. med. infect. dis.
• Založnik: MDPI
• ISSN: 2414-6366
• COBISS.SI-ID: 526463769

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: Escherichia coli, izolati, spodnje dihalne poti, β-laktamaze, razširjeno delovanje, rezistenčni geni, QAC

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P1-0198
• Naslov: Molekularno-biološke raziskave mikroorganizmov

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Program financ.: Young researchers