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Comparative analysis of DNA preservation in permanent and deciduous teeth of adults and non-adults : implications for archaeological and forensic research
ID
Leskovar, Tamara
(
Author
),
ID
Zupanič-Pajnič, Irena
(
Author
)
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https://www.sciencedirect.com/science/article/pii/S0379073823003328
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Abstract
This study investigates the preservation of DNA in different categories of teeth, including permanent and deciduous, fully developed and not fully developed, in both adults and non-adults. Teeth were sampled from a modern-era cemetery in Ljubljana, Slovenia. DNA extraction was performed using a full demineralisation protocol. DNA quantity and quality were assessed using qPCR analyses, and autosomal STR typing was conducted to verify genetic profiles. Results revealed significant differences in DNA preservation among various tooth categories. Fully developed permanent teeth of adults exhibited the highest DNA yields, attributed to their fully developed roots and thicker cementum, which is rich in DNA. Deciduous teeth, with thinner enamel and cementum, showed lower DNA preservation regardless of developmental stage. Non-adult teeth generally yielded less DNA compared to adults, even when considering only fully developed permanent teeth, indicating factors beyond developmental stage. These findings suggest that, in archaeological and forensic contexts, researchers should prioritize fully developed permanent teeth for DNA analysis due to their superior preservation. Additionally, this study underscores the importance of considering tooth type and developmental stage when selecting samples for genetic analysis in cases where petrous bone is unavailable, expanding our understanding of DNA preservation in human remains.
Language:
English
Keywords:
teeth
,
analysis of DNA preservation
,
forensic research
,
archeological research
,
DNA preservation
,
tooth type
,
permanent teeth
,
deciduous teeth
,
skeletonized remains
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
FF - Faculty of Arts
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2023
Number of pages:
8 str.
Numbering:
Vol. 353, art. 111882
PID:
20.500.12556/RUL-152934
UDC:
61
ISSN on article:
1872-6283
DOI:
10.1016/j.forsciint.2023.111882
COBISS.SI-ID:
173613571
Publication date in RUL:
12.12.2023
Views:
678
Downloads:
37
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Record is a part of a journal
Title:
Forensic science international
Publisher:
Elsevier
ISSN:
1872-6283
COBISS.SI-ID:
23017945
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
zobje
,
analiza ohranjenosti DNA
,
forenzična raziskava
,
arheološka raziskava
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
J3-3080
Name:
Določitev predniškega porekla iz DNA za identifikacijske namene
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