Comparative analysis of DNA preservation in permanent and deciduous teeth of adults and non-adults : implications for archaeological and forensic research

Leskovar, Tamara (author), Zupanič-Pajnič, Irena (author)

This study investigates the preservation of DNA in different categories of teeth, including permanent and deciduous, fully developed and not fully developed, in both adults and non-adults. Teeth were sampled from a modern-era cemetery in Ljubljana, Slovenia. DNA extraction was performed using a full demineralisation protocol. DNA quantity and quality were assessed using qPCR analyses, and autosomal STR typing was conducted to verify genetic profiles. Results revealed significant differences in DNA preservation among various tooth categories. Fully developed permanent teeth of adults exhibited the highest DNA yields, attributed to their fully developed roots and thicker cementum, which is rich in DNA. Deciduous teeth, with thinner enamel and cementum, showed lower DNA preservation regardless of developmental stage. Non-adult teeth generally yielded less DNA compared to adults, even when considering only fully developed permanent teeth, indicating factors beyond developmental stage. These findings suggest that, in archaeological and forensic contexts, researchers should prioritize fully developed permanent teeth for DNA analysis due to their superior preservation. Additionally, this study underscores the importance of considering tooth type and developmental stage when selecting samples for genetic analysis in cases where petrous bone is unavailable, expanding our understanding of DNA preservation in human remains.

• Language: English
• Keywords: teeth, analysis of DNA preservation, forensic research, archeological research, DNA preservation, tooth type, permanent teeth, deciduous teeth, skeletonized remains
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FF - Faculty of Arts; MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 8 str.
• Numbering: Vol. 353, art. 111882
• PID: 20.500.12556/RUL-152934
• UDC: 61
• ISSN on article: 1872-6283
• DOI: 10.1016/j.forsciint.2023.111882
• COBISS.SI-ID: 173613571
• Publication date in RUL: 12.12.2023

Record is a part of a journal

• Title: Forensic science international
• Publisher: Elsevier
• ISSN: 1872-6283
• COBISS.SI-ID: 23017945

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: zobje, analiza ohranjenosti DNA, forenzična raziskava, arheološka raziskava

Projects

• Funder: ARRS - Slovenian Research Agency
• Project number: J3-3080
• Name: Določitev predniškega porekla iz DNA za identifikacijske namene