20.500.12556/RUL-152934 Comparative analysis of DNA preservation in permanent and deciduous teeth of adults and non-adults implications for archaeological and forensic research This study investigates the preservation of DNA in different categories of teeth, including permanent and deciduous, fully developed and not fully developed, in both adults and non-adults. Teeth were sampled from a modern-era cemetery in Ljubljana, Slovenia. DNA extraction was performed using a full demineralisation protocol. DNA quantity and quality were assessed using qPCR analyses, and autosomal STR typing was conducted to verify genetic profiles. Results revealed significant differences in DNA preservation among various tooth categories. Fully developed permanent teeth of adults exhibited the highest DNA yields, attributed to their fully developed roots and thicker cementum, which is rich in DNA. Deciduous teeth, with thinner enamel and cementum, showed lower DNA preservation regardless of developmental stage. Non-adult teeth generally yielded less DNA compared to adults, even when considering only fully developed permanent teeth, indicating factors beyond developmental stage. These findings suggest that, in archaeological and forensic contexts, researchers should prioritize fully developed permanent teeth for DNA analysis due to their superior preservation. Additionally, this study underscores the importance of considering tooth type and developmental stage when selecting samples for genetic analysis in cases where petrous bone is unavailable, expanding our understanding of DNA preservation in human remains. teeth analysis of DNA preservation forensic research archeological research DNA preservation tooth type permanent teeth deciduous teeth skeletonized remains zobje analiza ohranjenosti DNA forenzična raziskava arheološka raziskava true false true Angleški jezik Slovenski jezik Članek v reviji 2023-12-12 13:45:14 2023-12-12 13:45:19 2023-12-13 03:41:48 0000-00-00 00:00:00 2023 0 0 8 str. art. 111882 Vol. 353 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 61 1872-6283 10.1016/j.forsciint.2023.111882 173613571 1-s2.0-S0379073823003328-main.pdf 1-s2.0-S0379073823003328-main.pdf 1 01378421A0721CC4FA2ED7DAEBD0336B b56f2c91b61c7f3546b88dc76bc7c878e351c4472f3c6ad1a292e29028c2caf5 b2dc3eb4-98ec-11ee-b821-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178679 https://www.sciencedirect.com/science/article/pii/S0379073823003328 0 4edadb72-98ec-11ee-b821-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=178678 Filozofska fakulteta Medicinska fakulteta 0 0 0