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Morphological characteristics of young and old murine hematopoietic stem cell niches, as modeled in vitro
ID
Justin, Mojca
(
Author
),
ID
Rogač Randl, Ema
(
Author
),
ID
Kononenko, Veno
(
Author
),
ID
Hočevar, Matej
(
Author
),
ID
Drobne, Damjana
(
Author
),
ID
Rožman, Primož
(
Author
)
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https://www.hindawi.com/journals/scanning/2023/5541050/
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Abstract
The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age-specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity, increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a complementary approach to imaging with specific cellular markers.
Language:
English
Keywords:
bone marrow
,
hematopoietic stem cell niches
,
aging
,
adipocytes
,
scanning electron microscopy
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
BF - Biotechnical Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2023
Number of pages:
10 str.
Numbering:
Vol. 2023, art. 5541050
PID:
20.500.12556/RUL-152339
UDC:
576
ISSN on article:
1932-8745
DOI:
10.1155/2023/5541050
COBISS.SI-ID:
149390083
Publication date in RUL:
21.11.2023
Views:
788
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38
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Record is a part of a journal
Title:
Scanning
Publisher:
Hindawi
ISSN:
1932-8745
COBISS.SI-ID:
517825049
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
kostni mozeg
,
niše hematopoetskih matičnih celic
,
staranje
,
adipociti
,
vrstična elektronska mikroskopija
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Funder:
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