20.500.12556/RUL-152339 Morphological characteristics of young and old murine hematopoietic stem cell niches, as modeled in vitro The hematopoietic stem cell (HSC) niche undergoes detrimental changes with age. The molecular differences between young and old niches are well studied and understood; however, young and old niches have not yet been extensively characterized in terms of morphology. In the present work, a 2D stromal model of young and old HSC niches isolated from bone marrow was investigated using light and scanning electron microscopy (SEM) to characterize cell density after one, two, or three weeks of culturing, cell shape, and cell surface morphological features. Our work is aimed at identifying morphological differences between young and old niche cells that could be used to discriminate between their respective murine HSC niches. The results show several age-specific morphological characteristics. The old niches differ from the young ones in terms of lower cell proliferating capacity, increased cell size with a flattened appearance, increased number of adipocytes, and the presence of tunneling nanotubes. In addition, proliferating cell clusters are present in the young niches but not in the old niches. Together, these characteristics could be used as a relatively simple and reliable tool to discriminate between young and old murine HSC niches and as a complementary approach to imaging with specific cellular markers. bone marrow hematopoietic stem cell niches aging adipocytes scanning electron microscopy kostni mozeg niše hematopoetskih matičnih celic staranje adipociti vrstična elektronska mikroskopija true false true Angleški jezik Slovenski jezik Članek v reviji 2023-11-21 10:31:55 2023-11-21 10:31:57 2023-11-22 03:38:21 0000-00-00 00:00:00 2023 0 0 10 str. art. 5541050 Vol. 2023 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 576 1932-8745 10.1155/2023/5541050 149390083 5541050.pdf 5541050.pdf 1 00DE5C1DF714EAAB8D34D099852A16EF 41d66ddfa9cf1b68e4251259270e2b6f51c2e31ce2bf6c601b9ac3d51cf551cd 540abc95-8851-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177800 https://www.hindawi.com/journals/scanning/2023/5541050/ 1 d0f8b865-8850-11ee-8d2e-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=177799 Biotehniška fakulteta 0 0 0