Your browser does not allow JavaScript!
JavaScript is necessary for the proper functioning of this website. Please enable JavaScript or use a modern browser.
Open Science Slovenia
Open Science
DiKUL
slv
|
eng
Search
Browse
New in RUL
About RUL
In numbers
Help
Sign in
Leptospirosis and characterization of Leptospira isolates from patients in Koprivnica-Križevci County, Croatia from 2000–2004
ID
Mišić-Majerus, Ljiljana
(
Author
),
ID
Cerar Kišek, Tjaša
(
Author
),
ID
Ružić-Sabljić, Eva
(
Author
)
PDF - Presentation file,
Download
(1,37 MB)
MD5: AEB0E67FCB4C0B3B3EE25DA9934827D8
URL - Source URL, Visit
https://www.microbiologyresearch.org/content/journal/acmi/10.1099/acmi.0.000431
Image galllery
Abstract
Introduction. Leptospirosis, caused by spirochetes of the genus Leptospira, is present in the Koprivnica-Križevci County area, Croatia. Clinical manifestation can range from asymptomatic, short-term mild, non-specific febrile disease, to severe forms with high mortality rates. Aim. The aim of the study was to valuate culture in front of microscopic agglutination test (MAT) for diagnosis of infection, and to evaluate clinical and laboratory features of the disease. Moreover, we want to characterize Leptospira strains involved in infection in Koprivnica-Križevci County, Croatia. Methods. We included 68patients with clinical presentation consistent with leptospirosis collected in a 5-year period (2000–2004). Clinical samples (blood, urine and cerebrospinal fluid, CSF) were inoculated in Kolthoff’s medium; species of isolated Leptospira strains was determined with Tm of real-time PCR, serogroup/serovar with MAT and NotI-RFLP analysis. Demonstration of specific antibodies in patients’ sera was done using microscopic agglutination test. Results. Leptospira was isolated from the blood of 14/51(27.5%) patients and the most often identified serogroup/serovar was Icterohaemorrhagiae (8/10, 80%) followed by Grippotyphosa (10%). Regarding to species level, 8/10 isolated belonged to L. interrogans sensu stricto and one to L. kirschneri species. MAT was carried out on 51patients with suspected leptospirosis, and was positive in 11/51(21.5%) patients. Most of our patients presented with moderate severe symptoms, were hospitalized from August to October, and were infected mainly during the work or recreation in our county. The frequency of particular clinical features and pathological laboratory findings correlated with the severity of the clinical condition. Conclusions. Leptospirosis can be confirmed microbiologically, culture and MAT contributed almost equally to the diagnosis of infection. Serovar Icterohaemorrhagiae was found as the dominant one, and L. interrogans sensu stricto as dominant species in our county. Epidemiological data shown that leptospirosis occurs seasonally, affects the rural population, and most commonly is presented with moderate severe clinical course.
Language:
English
Keywords:
leptospirosis
,
Leptospira isolation
,
Leptospira species
,
Leptospira serogroup
,
Leptospira serovar
,
MAT
,
Koprivnica-Križevci County
,
Croatia
,
MLST
,
sequence type
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2023
Number of pages:
12 str.
Numbering:
Vol. 5, iss. 4, art. 000431
PID:
20.500.12556/RUL-152336
UDC:
616.9:579
ISSN on article:
2516-8290
DOI:
10.1099/acmi.0.000431
COBISS.SI-ID:
168212995
Publication date in RUL:
21.11.2023
Views:
589
Downloads:
30
Metadata:
Cite this work
Plain text
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Copy citation
Share:
Record is a part of a journal
Title:
Access microbiology
Shortened title:
Access microbiol.
Publisher:
Microbiology Society
ISSN:
2516-8290
COBISS.SI-ID:
18872323
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
leptospiroza
,
Leptospira
,
izolati
,
Hrvaška
,
zaporedje vrste
,
mikrobiologija
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P3-0296
Name:
Bolezni in povzročitelji, ki jih v Sloveniji prenašajo členonožci
Similar documents
Similar works from RUL:
Similar works from other Slovenian collections:
Back