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Campylobacter magnus sp. nov., isolated from caecal contents of domestic pigs (Sus scrofa domesticus)
ID
Gruntar, Igor
(
Avtor
),
ID
Kostanjšek, Rok
(
Avtor
),
ID
Pirš, Tina
(
Avtor
),
ID
Papić, Bojan
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,44 MB)
MD5: 05431766F6189EB067762CE0A1301543
URL - Izvorni URL, za dostop obiščite
https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.006108
Galerija slik
Izvleček
During the 2021 European Food Safety Authority coordinated harmonized monitoring of antimicrobial resistance in Campylobacter species in Slovenia, five Campylobacter-like strains were cultured from caeca of a total of 104 domestic pigs that could not be identified using the standard-prescribed biochemical tests or MALDI-TOF MS. The isolates were obtained using the standard ISO 10272 procedure for the isolation of thermotolerant Campylobacter with prolonged cultivation time. Small Campylobacter like colonies were observed on mCCDA and CASA agar plates after 2–4days of incubation; dark-field microscopy revealed relatively big spirilli-shaped bacteria exhibiting characteristic Campylobacter-like motility. The cells were 1.5–3µm long and 0.5–0.7µm wide, Gram-negative, oxidase-positive and catalase-positive. MALDI-TOF mass spectra were distinctive and consistent, but with low MALDI-TOF MS log scores and the closest matches being those of Campylobacter hyointestinalis and Campylobacter fetus. All five strains underwent whole-genome sequencing. Analysis of 16S rRNA gene sequences revealed that the isolates were most similar (98.3–98.4% identity) to Campylobacter lanienae. Pairwise average nucleotide identity (ANI) values revealed that the five studied strains shared pairwise ANI of 96.2–96.5% but were clearly distinct from the previously described Campylobacter species (ANI ≤72.8%). The core genome-based phylogeny confirmed that the new strains form a distinct and well-supported clade within the genus Campylobacter. The conducted polyphasic taxonomic analysis confirmed that the five strains represent a novel Campylobacter species for which the name Campylobacter magnus sp. nov. is suggested, with strain 46386T (=DSM 115534T =CCUG 76865T ) as the type strain.
Jezik:
Angleški jezik
Ključne besede:
Campylobacter infections
,
swine
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
VF - Veterinarska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Datum objave:
01.01.2023
Leto izida:
2023
Št. strani:
Str. 1-7
Številčenje:
Vol. 73, iss. 10, [article no.] 006108
PID:
20.500.12556/RUL-152073
UDK:
636.09:616.9:579
ISSN pri članku:
1466-5034
DOI:
10.1099/ijsem.0.006108
COBISS.SI-ID:
170365443
Datum objave v RUL:
30.10.2023
Število ogledov:
438
Število prenosov:
58
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Objavi na:
Gradivo je del revije
Naslov:
International journal of systematic and evolutionary microbiology
Skrajšan naslov:
Int. j. syst. evol. microbiol.
Založnik:
Society for General Microbiology.
ISSN:
1466-5034
COBISS.SI-ID:
521767193
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Projekti
Financer:
ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
Številka projekta:
P4-0092
Naslov:
Zdravje živali, okolje in varna hrana
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