Simultaneous analysis of ascorbic and dehydroascorbic acid in fruit juice using HILIC chromatography coupled with mass spectrometry

Zia, Hassan (author), Fischbach, Nadine (author), Hofsommer, Mikko (author), Slatnar, Ana (author)

A HILIC-MS/MS method was developed for the simultaneous analysis of ascorbic acid (AA) and dehydroascorbic acid (DHA). Ammonium acetate buffer and acetonitrile were used as mobile phases under gradient elution mode at a flow rate of 0.3 mL/min. A SCIEX Triple QUAD 5500+ system was operated under MRM mode to acquire data with 175 → 115 and 173→113 as the quantifier ions for AA and DHA, respectively. Samples were prepared with a simple dilute and shoot method. A calibration and accuracy study was performed as part of method validation in fruit juices. The accuracy profiles were estimated from the measurement uncertainty determined from the validation data according to the ISO/DTS 21748. The recoveries of the analytes were between 92 – 130 %. The LOQs achieved in the current study for AA and DHA were 4.5 μg/L and 18 μg/L, respectively. These LOQs were substantially lower than what has been previously reported in the literature. The study concludes with a straightforward analytical method for the determination of total vitamin C content that does not involve timing consuming steps i.e., reduction or derivatization reaction. The method can be implemented in quality control labs for routine analysis of food samples.

• Language: English
• Keywords: HPLC–MS/MS, quality control, multifruit juice, fruit juices, ascorbic acid, dehydroascorbic acid, accuracy profile, validation
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 7 str.
• Numbering: Vol. 124, art. 105714
• PID: 20.500.12556/RUL-151767
• UDC: 543
• ISSN on article: 0889-1575
• DOI: 10.1016/j.jfca.2023.105714
• COBISS.SI-ID: 169061379
• Publication date in RUL: 19.10.2023

Record is a part of a journal

• Title: Journal of food composition and analysis
• Shortened title: J. food compos. anal.
• Publisher: Elsevier
• ISSN: 0889-1575
• COBISS.SI-ID: 25742848

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: sadni sok, vitamini, askorbinska kislina, dehidroaskorbinska kislina, kemična sestava, HPLC-MS/MS, kakovost

Projects

• Funder: EC - European Commission
• Funding programme: H2020
• Project number: 956257
• Name: Establishing a strong and lasting international training network for innovation in food and juice industries: a 4D-research approach for fruit juice processing
• Acronym: HiStabJuice