Simultaneous analysis of ascorbic and dehydroascorbic acid in fruit juice using HILIC chromatography coupled with mass spectrometry

Zia, Hassan (avtor), Fischbach, Nadine (avtor), Hofsommer, Mikko (avtor), Slatnar, Ana (avtor)

A HILIC-MS/MS method was developed for the simultaneous analysis of ascorbic acid (AA) and dehydroascorbic acid (DHA). Ammonium acetate buffer and acetonitrile were used as mobile phases under gradient elution mode at a flow rate of 0.3 mL/min. A SCIEX Triple QUAD 5500+ system was operated under MRM mode to acquire data with 175 → 115 and 173→113 as the quantifier ions for AA and DHA, respectively. Samples were prepared with a simple dilute and shoot method. A calibration and accuracy study was performed as part of method validation in fruit juices. The accuracy profiles were estimated from the measurement uncertainty determined from the validation data according to the ISO/DTS 21748. The recoveries of the analytes were between 92 – 130 %. The LOQs achieved in the current study for AA and DHA were 4.5 μg/L and 18 μg/L, respectively. These LOQs were substantially lower than what has been previously reported in the literature. The study concludes with a straightforward analytical method for the determination of total vitamin C content that does not involve timing consuming steps i.e., reduction or derivatization reaction. The method can be implemented in quality control labs for routine analysis of food samples.

• Jezik: Angleški jezik
• Ključne besede: HPLC–MS/MS, quality control, multifruit juice, fruit juices, ascorbic acid, dehydroascorbic acid, accuracy profile, validation
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 7 str.
• Številčenje: Vol. 124, art. 105714
• PID: 20.500.12556/RUL-151767
• UDK: 543
• ISSN pri članku: 0889-1575
• DOI: 10.1016/j.jfca.2023.105714
• COBISS.SI-ID: 169061379
• Datum objave v RUL: 19.10.2023

Gradivo je del revije

• Naslov: Journal of food composition and analysis
• Skrajšan naslov: J. food compos. anal.
• Založnik: Elsevier
• ISSN: 0889-1575
• COBISS.SI-ID: 25742848

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: sadni sok, vitamini, askorbinska kislina, dehidroaskorbinska kislina, kemična sestava, HPLC-MS/MS, kakovost

Projekti

• Financer: EC - European Commission
• Program financ.: H2020
• Številka projekta: 956257
• Naslov: Establishing a strong and lasting international training network for innovation in food and juice industries: a 4D-research approach for fruit juice processing
• Akronim: HiStabJuice