Enzymes play a crucial role in industry as environmentally friendly
biocatalysts. Immobilization can extend the time they can be used in processes, improve
stability under harsh industrial conditions and enable continuous use. Since enzymes also
need to be sufficiently pure, they need to be isolated and purified, before immobilization,
which is a challenging and costly step. This can be avoided by one-step immobilization
of enzymes. A common technique of direct enzyme immobilization is via tags. Such
technique are highly specific, as only the tagged enzymes bond on the carrier. Although
the activity of immobilized enzymes is lower than that of free enzymes, immobilized
biocatalysts can be used repeatedly and can be easily separated from the reaction mixture.
In this thesis a literature review of one-step purification and immobilization of enzymes
is presented.
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