20.500.12556/RUL-149728 Enostopenjsko čiščenje in imobilizacija encimov One-step purification and immobilization of enzymes Encimi imajo v industriji pomembno vlogo kot okolju prijazni biokatalizatorji. Z imobilizacijo lahko podaljšamo čas njihove uporabe v procesih, izboljšamo stabilnost pri ostrih industrijskih pogojih in izvajamo kontinuirne procese. Ker pa je potrebna tudi dovolj visoka čistost encimov, jih je pred tem potrebno izolirati in očistiti, kar pa predstavlja zahteven in drag korak. Temu se lahko izognemo z enostopenjsko imobilizacijo encimov. Pogosta tehnika direktne imobilizacije encimov je vezava preko označevalcev. Takšne tehnike so visoko specifične, saj vez z nosilcem tvorijo le označeni encimi. Aktivnost imobiliziranih encimov je sicer nižja od aktivnosti prostih, vendar pa imobilizirane biokatalizatorje lahko uporabimo večkrat in jih lažje ločimo od reakcijske mešanice. V diplomski nalogi je predstavljen pregled literature na področju enostopenjskega čiščenja in imobilizacije encimov. Enzymes play a crucial role in industry as environmentally friendly biocatalysts. Immobilization can extend the time they can be used in processes, improve stability under harsh industrial conditions and enable continuous use. Since enzymes also need to be sufficiently pure, they need to be isolated and purified, before immobilization, which is a challenging and costly step. This can be avoided by one-step immobilization of enzymes. A common technique of direct enzyme immobilization is via tags. Such technique are highly specific, as only the tagged enzymes bond on the carrier. Although the activity of immobilized enzymes is lower than that of free enzymes, immobilized biocatalysts can be used repeatedly and can be easily separated from the reaction mixture. In this thesis a literature review of one-step purification and immobilization of enzymes is presented. biokatalizator imobilizacija izolacija encimov biocatalyst immobilization enzyme isolation true false false Slovenski jezik Angleški jezik Diplomsko delo/naloga 2023-09-08 13:45:00 2023-09-08 13:45:00 2023-11-10 10:50:53 0000-00-00 00:00:00 2023 0 0 0000-00-00 NiDoloceno NiDoloceno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 23003 171642627 Verce_Andraz_-_Enostopenjsko_ciscenje_in_imobilizacija_encimov.pdf Verce_Andraz_-_Enostopenjsko_ciscenje_in_imobilizacija_encimov.pdf 1 79F47BE727791A5AEBA1DC4CBD84B270 d8da184e58e8767d35f218a58eccd284c57100cb26fdf769bce774dd590e72ef 23da691b-4e3d-11ee-9323-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=174228 Fakulteta za kemijo in kemijsko tehnologijo 0 0 0