Single-domain antibodies (nanobodies) have gained popularity in the last decade due to their stability and simple structure. Consisting of only one polypeptide chain, they can be synthesized by bacteria or yeasts as recombinant proteins, and still fold correctly. This allows us to create production systems which are relatively cheap and accessible. Yeast Saccharomyces cerevisiae belongs to GRAS organisms, thus allowing their protein products to have a broad range of applications – including in pharmacy. Upon transforming yeast cells with a plasmid containing a gene for a nanobody against the SARS CoV 2 spike protein following a gene for the alpha-factor leader peptide, the production under the control of an inducible promoter was induced, then the nanobody was isolated. Success of protein expression and secretion into the medium was evaluated using Western blot. In this research, we show that the suggested model system is applicable for production of the chosen nanobody.
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