Allergie to sertain plant based food is common problem for many people, who have to avoid foods that could potentially cause an allergic reaction and exclude them from their diet. Biotechnology and genetic engineering techniques such as RNAi and CRISPR/Cas9 offer possibility for hypoallergenic plant production, which would not trigger an allergic reaction even in sensitive individuals. Therefore they would potentially be able to consume them. The RNAi system is suitable for target silencing of gene expression that encode allergene proteins therefore it has already been used for silencing of allergene expression in soy, tomato, apple and peanut. However, recently CRISPR/Cas9 plays leading role in genetic engineering, as it is suitable for target genome editing and has been used for simultanious silencing of two soybean allergene expression. It is important to completely eliminate allergenes from plant based food, which requires an understanding of allergenic protein funcion in plant cell. Furthermore measurment of allergene reduction in hypoallergenic plant is necessary. This is possible through RNA and protein level quantification, vitally important are also clinical tests to determine response of sensitive individuals upon contact with hypoallergenic plant.
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