Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity

Grilc, Nina Katarina (author), Zidar, Anže (author), Kocbek, Petra (author), Rijavec, Tomaž (author), Colja, Teja (author), Lapanje, Aleš (author), Jeras, Matjaž (author), Gobec, Martina (author), Mlinarič-Raščan, Irena (author), Gašperlin, Mirjana (author), Kristl, Julijana (author), Zupančič, Špela (author)

Biofilm-associated diseases such as periodontitis are widespread and challenging to treat which calls for new strategies for their effective management. Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response. In this interdisciplinary study, nanofibers with two autochthonous Bacillus strains 27.3.Z and 25.2.M were developed. The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics. Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers. The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature. Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release. All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics. The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro. Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines. In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites.

• Language: English
• Keywords: probiotics, biofilm-related disease, periodontal disease, bacteria encapsulation, electrospinning, viability, release, erosion
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FFA - Faculty of Pharmacy
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: Str. 371-384
• Numbering: Vol. 355
• PID: 20.500.12556/RUL-148322-fbc49374-2873-e3d6-0d2c-901b44a9d7c9
• UDC: 66.017-022.513.2:616.311.2
• ISSN on article: 0168-3659
• DOI: 10.1016/j.jconrel.2023.01.082
• COBISS.SI-ID: 141431811
• Publication date in RUL: 11.08.2023

Record is a part of a journal

• Title: Journal of controlled release
• Shortened title: J. control. release
• Publisher: Elsevier
• ISSN: 0168-3659
• COBISS.SI-ID: 27318784

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: z biofilmom povezane bolezni, inkapsulacija bakterij, elektropredenje, preživetje, sproščanje, erozija, nanovlakna, probiotiki, parodontalne bolezni