20.500.12556/RUL-148322 Nanofibers with genotyped Bacillus strains exhibiting antibacterial and immunomodulatory activity Biofilm-associated diseases such as periodontitis are widespread and challenging to treat which calls for new strategies for their effective management. Probiotics represent a promising approach for targeted treatment of dysbiosis in biofilm and modulation of host immune response. In this interdisciplinary study, nanofibers with two autochthonous Bacillus strains 27.3.Z and 25.2.M were developed. The strains were isolated from the oral microbiota of healthy individuals, and their genomes were sequenced and screened for genes associated with antimicrobial and immunomodulatory activities, virulence factors, and transferability of resistance to antibiotics. Spores of two Bacillus strains were incorporated individually or in combination into hydrophilic poly(ethylene oxide) (PEO) and composite PEO/alginate nanofibers. The nanofiber mats were characterised by a high loading of viable spores (> 7 log CFU/mg) and they maintained viability during electrospinning and 6 months of storage at room temperature. Spores were rapidly released from PEO nanofibers, while presence of alginate in the nanofibers prolonged their release. All formulations exhibited swelling, followed by transformation of the nanofiber mat into a hydrogel and polymer erosion mediating spore release kinetics. The investigated Bacillus strains released metabolites, which were not cytotoxic to peripheral blood mononuclear cells (PBMCs) in vitro. Moreover, their metabolites exhibited antibacterial activity against two periodontopathogens, an antiproliferative effect on PBMCs, and inhibition of PBMC expression of proinflammatory cytokines. In summary, the developed nanofiber-based delivery system represents a promising therapeutic approach to combat biofilm-associated disease on two fronts, namely via modulation of the local microbiota with probiotic bacteria and host immune response with their metabolites. probiotics biofilm-related disease periodontal disease bacteria encapsulation electrospinning viability release erosion z biofilmom povezane bolezni inkapsulacija bakterij elektropredenje preživetje sproščanje erozija nanovlakna probiotiki parodontalne bolezni true false true Angleški jezik Slovenski jezik Članek v reviji 2023-08-11 15:07:08 2023-08-11 15:07:12 2025-08-08 13:22:32 0000-00-00 00:00:00 2023 0 0 Str. 371-384 Vol. 355 2023 0000-00-00 Zaloznikova Objavljeno NiDoloceno 0000-00-00 0000-00-00 0000-00-00 66.017-022.513.2:616.311.2 0168-3659 10.1016/j.jconrel.2023.01.082 141431811 1-s2.0-S0168365923000913-main.pdf 1-s2.0-S0168365923000913-main.pdf 1 62E1B906C33765D4CBDFA13B5EE831AB ad9596a0104049cb5be3d32bbb206a86f076040fd4622d1b47a0737c7f47a778 4d198933-3848-11ee-b9d7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172439 https://www.sciencedirect.com/science/article/pii/S0168365923000913 0 fb672e50-3847-11ee-b9d7-0050569b8976 https://repozitorij.uni-lj.si/Dokument.php?lang=slv&id=172438 Fakulteta za farmacijo 0 0 0